4种吴茱萸属植物叶片和果实的乙醇提取物及其体外抗肿瘤活性  

Ethanol extracts from leaves and fruits of four Evodia species and their in vitro antitumor activities

作　　者：靳桐 印敏[1] 管福琴[1] 李冬玲[2] 佟海英[2] 冯煦[1] 单宇[1] 王奇志[1] JIN Tong;YIN Min;GUAN Fuqin;LI Dongling;TONG Haiying;FENG Xu;SHAN Yu;WANG Qizhi(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences:Jiangsu Key Laboratory for the Research and Utilization of Plant Resources,Nanjing 210014,China;Institute of Botany,Jiangsu Province and Chinese Academy of Sciences:Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm,Nanjing 210014,China)

机构地区：[1]江苏省中国科学院植物研究所(南京中山植物园)江苏省植物资源研究与利用重点实验室,江苏南京210014 [2]江苏省中国科学院植物研究所(南京中山植物园)江苏省农业种质资源保护与利用平台,江苏南京210014

出　　处：《植物资源与环境学报》2021年第2期77-79,共3页Journal of Plant Resources and Environment

基　　金：江苏省林业科技创新与推广项目(LYKJ[2020]09);江苏省植物资源研究与利用重点实验室开放基金项目(JSPKLB201925,JSPKLB202051)。

摘　　要：吴茱萸属(Evodia J.R.et G.Forst.)植物的提取物及单体化合物不仅具有镇痛抗炎[1]、改善胃肠道功能[2]、保护心血管[3]和治疗阿尔茨海默病[4]等作用,还具有抑制乳腺癌[5]、结肠癌[6]、肝癌[7]和黑色素瘤[8]等肿瘤相关疾病的药理活性。目前,对吴茱萸属植物的研究主要集中在《中华人民共和国药典》中收录的吴茱萸〔E.rutaecarpa(Juss.)Benth.〕、石虎〔E.rutaecarpa var.officinalis(Dode)Huang〕和疏毛吴茱萸〔E.rutaecarpa var.bodinieri(Dode)Huang〕3种基源植物[9]。Total extracts of 80%(volume fraction)ethanol(hereinafter referred to as total extracts)and its dichloromethane,ethyl acetate,and water fractions were obtained from leaves and fruits of four Evodia J.R.et G.Forst.species by using reflux extraction and solvent extraction,and their in vitro antitumor activities and toxicities were evaluated by using MTT method.The results show that the extraction rates of total extracts from leaves and fruits of E.ailanthifolia Pierre,E.austrosinensis Hand.-Mazz.,E.daniellii(Benn.)Hemsl.,and E.delavayi Dode are in the range of 13.00%-30.35%.The pharmacological experiment result shows that at the drug concentration of 10μg·mL-1,the inhibition rates of total extracts and its water fraction from leaves of E.ailanthifolia,ethyl acetate fraction of total extracts from fruits of E.austrosinensis,and total extracts from fruits of E.daniellii on breast cancer cell MDA-MB-231 and hepatoma cell HepG2 are higher than 50%,those of dichloromethane fraction of total extracts from leaves of E.ailanthifolia on three tumor cells are all higher than 50%,those of ethyl acetate fraction of total extracts from leaves of E.ailanthifolia on breast cancer cell MDA-MB-231 and colon cancer cell LoVo are higher than 50%,and their inhibition rates on the normal human aortic smooth muscle cell HASMC are lower than 40%.Therefore,above extracts have potential values as a source of natural antitumor drug.

关 键 词：吴茱萸属 抗肿瘤活性 MTT法 毒性评价 

分 类 号：Q946[生物学—植物学] R284.2[医药卫生—中药学]