IL-6/STAT3信号通路在百草枯中毒急性肺损伤中作用机制的研究  

Study on the mechanism of IL-6/STAT3 signaling pathway in acute lung injury induced by paraquat poisoning

作　　者：李激文 钟雨 刘华[1] 蔡能斌[1] 黄俊杰 韦雪花 邓旺生[1] 黎宇[1] LI Jiwen;ZHONG Yu;LIU Hua;CAI Nengbin;HUANG Junjie;WEI Xuehua;DENG Wangsheng;LI Yu(Emergency Department,Liuzhou Workers'Hospital,Liuzhou 545005,Guangxi,China)

机构地区：[1]广西柳州市工人医院急诊科,广西柳州545005

出　　处：《右江医学》2021年第3期172-177,共6页Chinese Youjiang Medical Journal

基　　金：广西急诊与医学救援人才小高地、广西高校急诊医学重点实验室开放课题(GXJZ201623);广西卫生和计划生育委员会自筹经费课题(Z2016181)。

摘　　要：目的探讨IL-6/STAT3信号通路在百草枯中毒急性肺损伤中的作用。方法选取72例百草枯中毒患者,根据Murray肺损伤评分分为无肺损伤组、轻微-中度肺损伤组、重度肺损伤组,每组24例,同时收集22名健康者作为对照。支气管肺泡灌洗、AM分离及培养后,ELISA检测血清IL-6、sIL-6R、sgp130的蛋白表达,Westernblot检测STAT3、pSTAT3、gp130的蛋白表达,RT-PCR分析STAT3、gp130mRNA表达。结果轻微-中度肺损伤组、重度肺损伤组IL-6、sIL-6R、sgp130、STAT3、pSTAT3、gp130的蛋白表达明显高于无肺损伤组、对照组(P<0.05),且重度肺损伤组IL-6、sIL-6R、sgp130、STAT3、pSTAT3、gp130蛋白表达水平明显高于轻微-中度肺损伤组(P<0.05);重度肺损伤组STAT3mRNA、gp130mRNA表达高于其他三组(P<0.05)。结论百草枯中毒肺损伤患者中IL-6/STAT3信号通路通过上调sgp130、gp130蛋白的表达,升高炎症反应程度,对肺组织造成损伤。Objective To investigate the role of IL-6/STAT3 signaling pathway in acute lung injury induced by paraquat poisoning.Methods 72 patients with paraquat poisoning were selected,and they were divided into three groups according to Murray lung injury score:non lung injury group,mild to moderate lung injury group and severe lung injury group,with 24 cases in each group,and other 22 healthy people in the same period were selected as control group.Then,after bronchoalveolar lavage,AM isolation and culture,the protein expressions of serum IL-6,sIL-6R and sgp130 were detected by ELISA,the protein expressions of STAT3,pSTAT3 and gp130 were detected by Western blot,and the mRNA expressions of STAT3 and gp130 were analyzed by RT-PCR.Results The protein expressions of IL-6,sIL-6R,sgp130,STAT3,pSTAT3 and gp130 in the mild to moderate lung injury group and severe lung injury group were higher than those in the non lung injury group and the control group(P<0.05),and the expression levels of IL-6,sIL-6R,sgp130,STAT3,pSTAT3 and gp130 protein in the severe lung injury group were higher than those in the mild to moderate lung injury group(P<0.05).In addition,STAT3mRNA and gp130mRNA expressions in the severe lung injury group were higher than those in the other three groups(P<0.05).Conclusion IL-6/STAT3 signaling pathway can increase the degree of inflammatory reaction by up regulating the expressions of sgp130 and gp130 protein in patients with lung injury induced by paraquat poisoning.

关 键 词：百草枯 急性肺损伤 肺泡灌洗 白介素-6 信号传导与转录激活因子3 

分 类 号：R595.4[医药卫生—内科学]