进境新西兰苹果中欧洲枝溃疡病菌的分离和鉴定  

Isolation and identification of Neonectria ditissima obtained from New Zealand apple fruit

作　　者：焦彬彬 马纯珏 何善勇 孙涛 杨翠云 Jiao Bin-bin;Ma Chunjue;He Shanyong;Sun Tao;Yang Cuiyun(Key Laboratory of Southwest China Wildlife Resources Conservation,Ministry of Education//Collage of Life Science,West Normal University,Nanchong 637009,China;Shanghai Customs District;Chongqing Customs District)

机构地区：[1]西华师范大学生命科学院西南野生动植物资源保护教育部重点实验室,室四川南充637009 [2]上海海关 [3]重庆海关

出　　处：《植物检疫》2021年第2期33-38,共6页Plant Quarantine

基　　金：海关总署科研项目(2019HK044);国家重点研发计划国家质量基础(NQI)专项(2017YFF0210302)。

摘　　要：从新西兰进境苹果的褐腐组织中分离得到1株新丛赤壳属菌株3072,对该菌株进行形态学特征观察、PCR检测、序列比对分析和致病性测定。结果表明,在PDA培养基上菌落乳白色至黄白色,气生菌丝茂密,边缘规则;在MEA培养基上菌落为白色、平铺生长。菌株为异宗配合。利用欧洲枝溃疡病菌(Neonectria ditissima)特异检测引物对Bt-fw135/Bt-rw284扩增菌株的基因组DNA得到150 bp的预期目标条带。菌株的β-tubulin序列与GenBank中N.ditissima的序列相似性为99.43%100%。基于β-tubulin序列构建的系统进化树显示菌株3072与N.ditissima聚在同一个分支,亲缘关系最近。菌株接种苹果,接种处组织14 d后出现典型褐腐症状。根据上述检测结果,将菌株3072鉴定为欧洲枝溃疡病菌(N.ditissima)。An isolate 3072 similar to Neonectria spp.was obtained from the brown rot tissue of imported apple fruit from New Zealand.To identify the isolate,its morphological characteristics,PCR detection with specific primers,β-tubulin sequence and its pathogenicity were analyzed.The data showed that colonies were milky or yellowish white with abundant aerial mycelium and regular margin on PDA medium;white and flat on MEA medium.The isolate is heterothallic.The result of PCR detection showed that the isolate 3072 can be amplified by N.ditissima specific primers Bt-fw135/Bt-rw284 and got expected bands of 150 bp.β-tubulin sequence of the isolate showed 99.43%-100%identity with N.ditissimaβ-tubulin sequences deposited in GenBank.The phylogenetic tree of theβ-tubulin sequences showed the isolate 3072 was clustered closely with N.ditissima.Typical symptom of brown rot was observed on the wounded-inoculation apple after 14 days.All the results showed that the isolate 3072 was N.ditissima.

关 键 词：苹果 欧洲枝溃疡病菌 鉴定 

分 类 号：S41-33[农业科学—植物保护]