黄芩透过人胎盘屏障体外模型药物成分研究  

Study on ingredients of Scutellaria baicalensis through human placental barrier

作　　者：鲁娣 张崴[1] 郭洁[1] 王雅楠[1] 杜文欣[1] 宋殿荣[1] LU Di;ZHANG Wei;GUO Jie;WANG Ya-nan;DU Wen-xin;SONG Dian-rong(Department of Gynecology,Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300150,China)

机构地区：[1]天津中医药大学第二附属医院妇科,天津300150

出　　处：《中国临床药理学杂志》2021年第6期676-680,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81573740)。

摘　　要：目的研究黄芩透过人胎盘屏障药物成分。方法用尤斯灌流室技术建立人胎盘屏障体外模型,预平衡30 min后,于母体池加入10 mg·mL-1黄芩溶液,分别于5~240 min内在胎儿池取样,用高效液相色谱技术分析样品中所含药物成分。色谱柱:Agilent Zorbax-C18(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%甲酸水(梯度洗脱),内标为葛根素,流速为1.0 mL·min^(-1),检测波长为278 nm,柱温20℃,进样量为5μL。结果从5 min开始,黄芩苷、汉黄芩苷、汉黄芩素即可透过人胎盘屏障;黄芩素和千层纸素A从20 min开始才透过胎盘屏障;30 min后检测到的11种药物成分均可透过胎盘屏障,且在后续取样时间点未见新的物质生成。结论黄芩中的5种黄酮类成分(黄芩苷、汉黄芩苷、黄芩素、汉黄芩素和千层纸素A)均可透过人胎盘屏障体外模型。Objective To study the ingredients of Scutellaria baicalensis through human placental barrier.Methods A human placental barrier in vitro model was established by Ussing chamber,then different concentrations of Scutellaria baicalensis(10 mg·mL-1)were added in maternal sides after balance for 30 min.At the time of 5-240 min after dosing in fatal sides,the ingredients were analyzed using high performance liquid chromatography(HPLC)technique.HPLC was performed on a column of Agilent Zorbax-C18(4.6 mm×250 mm,5μm)with mobile phase of acetonitrile-0.1%formic acid water(gradient elution),the internal standard was puerarin,the flow rate was 1.0 mL·min^(-1),and the detection wavelength was 278 nm,the column temperature was 20℃,and the injection volume was 5μL.Results With 5 min,baicalin,wogonoside,and wogonin can pass through the human placental barrier;baicalein and laminin A only pass through the placental barrier from 20 min;after 30 min all of 11 components detected pass through the placental barrier,no new material was found at subsequent sampling points.Conclusion Baicalin,wogonoside,baicalein,wogonin,and oroxylin-A in Scutellaria baicalensis can pass through the human placental barrier model in vitro.

关 键 词：黄芩 高效液相色谱法 胎盘屏障 黄芩苷 汉黄芩苷 黄芩素 汉黄芩素 千层纸素A 

分 类 号：R28[医药卫生—中药学]