长链非编码RNA ANCR在肺癌中的表达和作用  

Expression and role of long noncoding RNA ANCR in lung cancer

作　　者：陈辉国 周亚夫[1] 颜建华[1] 肖国华 田双如 杨劲松[1] CHEN Hui-guo;ZHOU Ya-fu;YAN Jian-hua;XIAO Guo-hua;TIAN Shuang-ru;YANG Jin-song(Department of Cardiothoracic Surgery,Hunan People's Hospital/the First Affiliated Hospital of Hunan Normal University,Changsha 410005,Hunan Province,China)

机构地区：[1]湖南省人民医院、湖南省师范大学附属第一医院心胸外科,湖南长沙410005

出　　处：《中国临床药理学杂志》2021年第6期685-689,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探讨长链非编码RNA ANCR(LncRNA ANCR)在肺癌组织中的表达及对肺癌细胞增殖和凋亡的作用。方法收集50例肺癌患者的肺癌组织和癌旁组织,用实时荧光定量逆转录-聚合酶链反应(RT-qPCR)检测组织中LncRNA ANCR和叉头框蛋白O1(FOXO1)mRNA的表达水平。将A549细胞分为第1转染组(转染慢病毒阴性对照质粒)、第2转染组(转染pHRi-ANCR)、第3转染组(转染pHRi-sh-ANCR)、第4转染组(转染pHRi-FOXO1)和第5转染组(同时转染pHRi-sh-ANCR和pHRi-sh-FOXO1),未处理细胞为空白组。用RNA pull-down和RIP实验检测ANCR与FOXO1的相互作用。用RT-qPCR检测ANCR和FOXO1的表达水平,用EdU标记技术和流式细胞术分别检测A549细胞增殖、细胞周期和细胞凋亡情况。结果肺癌组织和癌旁组织中LncRNA ANCR的表达分别为2.78±1.36,1.33±0.51,FOXO1 mRNA的表达为1.28±0.57,3.03±0.72。第1,3,4,5转染组细胞增殖比例分别为(47.90±2.36)%,(27.93±2.37)%,(29.93±0.64)%,(74.27±4.06)%,阻滞于G0/G1期细胞比例分别为(37.43±1.96)%,(64.20±4.05)%,(65.47±1.86)%,(24.93±1.38)%,细胞凋亡比例分别为(8.93±0.53)%,(17.81±1.42)%,(16.18±1.07)%,(7.78±0.55)%。肺癌组织与癌旁组织相比、第3,4转染组与第1转染组相比、第5转染组与第3转染组相比,差异均具有统计学意义(均P<0.05)。结论LncRNA ANCR在肺癌中表达升高并通过抑制FOXO1的表达调控肺癌细胞的细胞增殖和凋亡。Objective To investigate the expression of long noncoding RNA ANCR(LncRNA ANCR)in lung cancer tissues and its effect on proliferation and apoptosis of lung cancer cells.Methods The expression levels of LncRNA ANCR and Forkhead box class O1(FOXO1)mRNA in lung cancer tissues and adjacent tissues of 50 lung cancer patients were detected by reverse transcription-quantitiative polymerase chain reaction(RT-qPCR).A549 cells were divided into the Transfection-1 group(transfected with lentivirus-negative control plasmid),the Transfection-2 group(transfected with pHRi-ANCR),the Transfection-3 group(transfected with pHRi-sh-ANCR),the Transfection-4 group(transfected with pHRi-FOXO1)and the Transfection-5 group group(transfected with both pHRi-sh-ANCR and pHRi-sh-FOXO1),and the untreated cells were the blank group.The interaction between ANCR and FOXO1 was detected by RNA pull-down and RIP experiments.The expression levels of ANCR and FOXO1 were detected by RT-qPCR,and the proliferation,cell cycle and apoptosis of A549 cells were detected by EdU labeling and flow cytometry,respectively.Results The expression of LncRNA ANCR were2.78±1.36,1.33±0.51,and the expression of FOXO1 mRNA were 1.28±0.57,3.03±0.72 in lung cancer and adjacent tissues,respectively.The cell proliferation rates of Transfection-1,Transfection-3 group,Transfection-4 group and Transfection-5 group were(47.90±2.36)%,(27.93±2.37)%,(29.93±0.64)%,(74.27±4.06)%.The cell arrest in G0/G1 phase rates were(37.43±1.96)%,(64.20±4.05)%,(65.47±1.86)%,(24.93±1.38)%.The apoptosis rates were(8.93±0.53)%,(17.81±1.42)%,(16.18±1.07)%,(7.78±0.55)%.The differences between lung cancer tissues and adjacent tissues,between the Transfection-3,Transfection-4 group and the Transfection-1 group,and between the Transfection-5 group and the Transfection-3 group were all statistically significant(P<0.05).Conclusion LncRNA ANCR expression is increased in lung cancer and regulates cell proliferation and apoptosis of lung cancer cells by inhibiting FOXO1 expression.

关 键 词：长链非编码RNA ANCR 叉头框蛋白O1 肺癌 细胞增殖 细胞周期 细胞凋亡 

分 类 号：R97[医药卫生—药品]