基于PI3K/AKT/GSK-3β信号通路探讨鳖甲煎丸调控肝癌细胞Hep3B增殖转移的机制  

Mechanism research of regulation of proliferation and metastasis of hepatoma cells Hep3B by Biejiajian Pill based on PI3K/AKT/GSK-3β signaling pathway

作　　者：孙嘉玲 文彬 杨雪梅[1] 陈炜聪 招文婷 孙海涛[1] 陈冠新[1] 腊蕾[3] 贺松其[1] SUN Jia-ling;WEN Bin;YANG Xue-mei;CHEN Wei-cong;ZHAO Wen-ting;SUN Hai-tao;CHEN Guan-xin;LA Lei;HE Song-qi(School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China;Department of Traditional Chinese Medicine,The Air Force Hospital of Southern Theater Command,Guangzhou 510602,China;Department of Pharmacy,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]南方医科大学中医药学院,广州510515 [2]中国人民解放军南部战区空军医院中医科,广州510602 [3]南方医科大学南方医院药学部,广州510515

出　　处：《中华中医药杂志》2021年第3期1361-1365,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81573808)。

摘　　要：目的:研究鳖甲煎丸对肝癌细胞Hep3B增殖和转移的影响,探讨其抗肝细胞癌的作用机制。方法:分别使用不同浓度的鳖甲煎丸药物血清培养肝癌细胞Hep3B,采用CCK8、细胞划痕实验观察肝癌细胞增殖和转移能力的改变;采用Western Blot检测PI3K、p-AKT、GSK-3β、p-GSK-3β、E-cadherin、N-cadherin、Snail蛋白表达的变化。结果:与NC组比较,各给药组均能显著抑制肝癌细胞Hep3B的增殖和转移(P<0.05),p-AKT、p-GSK-3β蛋白表达均显著下调,GSK-3β蛋白表达水平均显著上调(P<0.05);E-cadherin蛋白表达水平均显著上调,而N-cadherin、Snail蛋白表达水平均显著下调(P<0.05);其中,H组可显著下调PI3K蛋白表达水平(P<0.05),其他各组变化不明显。结论:鳖甲煎丸可以显著抑制肝癌细胞Hep3B的增殖和转移,其机制可能与通过PI3K/AKT/GSK-3β信号通路调控Snail转录因子来调节上皮间质转化有关。Objective: To study the effect of Biejiajian Pill on the proliferation and metastasis of hepatoma cells Hep3 B, and to explore its anti-hepatocellular carcinoma mechanism. Methods: Hep3 B cells were cultured with different concentrations of Biejiajian Pill drug serum. CCK8 and wound healing assay were used to detect the proliferation and metastasis ability of hepatoma cells. The protein expression of PI3 K, p-AKT, GSK-3β, p-GSK-3β, E-cadherin, N-cadherin and Snail were detected by Western Blot. Results: Compared with the NC group, all giving drug group could significantly inhibit the proliferation and metastasis of Hep3 B cells(P<0.05), the protein expression of p-AKT and p-GSK-3β in each group were significantly down-regulated, and the protein expression of GSK-3β was significantly up-regulated(P<0.05), the protein expression of E-cadherin was significantly upregulated in each group, and the protein expression of N-cadherin, Snail were significantly down-regulated in each group(P<0.05), the protein expression of PI3 K was significantly decreased in the H group(P<0.05), but not significantly in the other groups. Conclusion: Biejiajian Pill can significantly inhibit the proliferation and metastasis of hepatoma cells Hep3 B, and the mechanism may be related to the regulation of Snail transcription factor by PI3 K/AKT/GSK-3β signaling pathway to regulate epithelialmesenchymal transition.

关 键 词：鳖甲煎丸 肝细胞癌 PI3K/AKT/GSK-3β信号通路 增殖 转移 上皮间质转化 机制 

分 类 号：R285[医药卫生—中药学]