慢性束缚应激模拟情志因素对多发性抽动症大鼠模型影响的评价  

Evaluation of the influence of chronic restraint stress simulated emotional factors on the rat model for Tourette syndrome

作　　者：张宏贤 郭璠 张雯 姚楠 周倩倩[1] 崔霞 ZHANG Hong-xian;GUO Fan;ZHANG Wen;YAO Nan;ZHOU Qian-qian;CUI Xia(Beijing University of Chinese Medicine,Beijing 100029,China;Fangshan District Maternal and Child Health Care Center,Beijing 102488,China;Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China;Xi’an Brain Disease Hospital of TCM,Xi’an 710068,China)

机构地区：[1]北京中医药大学,北京100029 [2]房山区妇幼保健院,北京102488 [3]北京中医药大学第三附属医院,北京100029 [4]西安中医脑病医院,西安710068 [5]北京中医药大学第三附属医院儿科,100029

出　　处：《中华中医药杂志》2021年第3期1383-1388,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京市自然科学基金项目(No.7182101);第四批全国中医优秀人才研修项目(No.201724);国家自然科学基金青年科学基金项目(No.81503615);北京中医药大学自主选题项目。

摘　　要：目的:验证慢性束缚应激多发性抽动症(TS)模型能否成为研究情志因素对TS影响的动物模型。方法:SD大鼠按体质量随机分组,TS组、束缚TS组予亚氨基二丙腈(IDPN)250mg·kg-1·d-1,空白组、束缚组予0.9%氯化钠溶液250mg·kg-1·d-1,均腹腔注射7d;束缚组、束缚TS组予慢性束缚21d,第22天进行行为学和神经递质检测。结果:束缚TS组、TS组刻板运动评分较空白组、束缚组显著升高(P<0.01)。TS组旷场实验活动总得分较空白组、束缚组均显著升高(P<0.01),束缚TS组活动总得分较TS组降低(P<0.05)。束缚TS组、TS组高架十字迷宫(EPM)实验OE/TE值较束缚组升高(P<0.05)。束缚TS组悬尾实验不动时间较其它3组有延长趋势。TS组皮质NE和5-HT含量较空白组显著升高(P<0.01),束缚TS组皮质5-HT含量较TS组降低(P<0.05);束缚TS组纹状体DA和5-HT含量较束缚组升高(P<0.05)。束缚TS组皮质GABA含量较空白组、TS组、束缚组均显著升高(P<0.01,P<0.05);束缚TS组和TS组纹状体GLU含量较束缚组降低(P<0.05,P<0.01)。束缚TS组纹状体GABA含量较束缚组降低(P<0.05)。结论:慢性束缚应激TS大鼠模型是研究情志因素对TS影响较为理想的动物模型,可供研究应用。Objective: To verify whether the chronic restraint stress Tourette syndrome(TS) model could be used as an animal model to study the influence of emotional factors on TS. Methods: SD rats were randomly assigned by weight. The TS group and the restrained TS group were intraperitoneally injected with IDPN, 250 mg · kg-1 · d-1 for 7 consecutive days. The control group and the restrained group were intraperitoneally injected with normal saline, 250 mg · kg-1 · d-1 for 7 consecutive days. The restrained group and the restrained TS group were given chronic restraint stimulation until the 21 st day. Conduct behavioral and neurotransmitter testing on the 22 nd day. Results: The stereotype scores of the restrained TS group and the TS group were significantly higher than those of the control group and restrained group(P<0.01). The total activity score of the TS group was significantly higher than that of the control group and the restrained group(P<0.01). The total activity score of the restrained TS group was lower than that of the TS group(P<0.05). In the restrained TS group, the TS group OE/TE value was higher than the restrained group(P<0.05). The immobility time of rats in the restrained TS group showed an increasing trend compared with that in the restrained group, the TS group, and the control group. The content of NE and 5-HT of the cortex in the TS group was significantly higher than that in the control group(P<0.01), the 5-HT content of the restrained TS group was lower than that of the TS group(P<0.05). The content of DA and 5-HT of corpus striatum in the restrained TS group was higher than that in the restrained group(P<0.05). The GABA content of the restrained TS group was significantly higher than that of the control group, the TS group and the restrained group(P<0.01, P<0.05). The GLU content of the restrained TS group and TS group was lower than that of the restrained group(P<0.05, P<0.01). The GABA content of the restrained TS group was significantly lower than that of the restrained group(P<0.05). Conc

关 键 词：慢性束缚应激 情志因素 多发性抽动症 多巴胺 5-羟色胺 Γ-氨基丁酸 行为学 动物模型 

分 类 号：R277.7[医药卫生—中医学] R-332