代谢综合征痰证与湿证患者的血清代谢组学比较研究  

Comparative study of serum metabolomics between phlegm syndrome and dampness syndrome of metabolic syndrome

作　　者：张萍[1,2,3] 李缘缘 邵岩飞 高碧珍 ZHANG Ping;LI Yuan-yuan;SHAO Yan-fei;GAO Bi-zhen(Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Research Base of Traditional Chinese Medicine Syndrome,Fuzhou 350122,China;Fujian Province 2011 TCM Health Management Collaborative Innovation Center,Fuzhou 350122,China;Key Laboratory of TCM Health Differentiation in Fujian Province,Fuzhou 350122,China)

机构地区：[1]福建中医药大学,福州350122 [2]中医证研究基地,福州350122 [3]福建省2011中医健康管理协同创新中心,福州350122 [4]福建省中医健康状态辨识重点实验室,福州350122

出　　处：《中华中医药杂志》2021年第3期1627-1632,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81873234,No.81273666);福建省自然科学基金面上项目(No.2018J01875);福建省2011中医健康管理协同创新中心资助项目(No.JG2017004-协同)。

摘　　要：目的:比较代谢综合征(MS)痰证与湿证患者血清代谢组学特征。方法:收集MS痰证患者30例、湿证患者30例,30名健康人群,运用核磁共振氢谱(1H-NMR)定量检测血清代谢物含量,采用主成分分析法(PCA)、正交偏最小二乘判别分析法(OPLS-DA)进行多元统计分析。结果:痰证的潜在标志物为胆碱、二甲胺、脂类=CH-CH2-CH-、肌氨酸、苏氨酸;湿证的潜在标志物为丙酮、乳酸、脂类-CH2-C=O、脂类-CH=CH-、丙酮酸、极低密度脂蛋白-CH2-CH2-C=O。结论:MS痰证、湿证患者血清存在物质代谢及代谢通路的改变,MS湿证患者可能存在糖代谢异常及脂代谢异常。Objective: To investigate the serum metabolomic characteristics of patients with phlegm and dampness syndrome of metabolic syndrome(MS). Methods: A total of 30 MS patients with phlegm syndrome, 30 MS patients with dampness syndrome and 30 healthy people were collected. Serum metabolites were quantitatively measured by 1 H-NMR. Multivariate statistical analysis was performed by PCA and OPLS-DA. Results: The potential markers of phlegm syndrome were choline, dimethylamine, lipids=CH-CH2-CH-, sarcosine and threonine. The potential markers of dampness syndrome were acetone, lactic acid, lipids,-CH2-C=O, lipids-CH=CH-, pyruvic acid, very low-density lipoprotein-CH2-CH2-C=O. Conclusion: MS patients with phlegm and dampness syndrome may have abnormal glucose metabolism and lipid metabolism.

关 键 词：代谢综合征 代谢组学 痰证 湿证 糖代谢 脂代谢 主成分分析法 正交偏最小二乘判别分析法 

分 类 号：R259[医药卫生—中西医结合]