膈下逐瘀汤加减方对气滞血瘀型输卵管炎大鼠血清IL-4、IL-8的影响  

Effects of modified Gexia Zhuyu Decoction on the serum IL-4 and IL-8 in rats with salpingitis with stagnation qi and blood stasis

作　　者：王亚文 胡喜姣 刘进哲 刘丽[3] WANG Ya-wen;HU Xi-jiao;LIU Jin-zhe;LIU Li(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China;JFirst Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,哈尔滨150040 [2]黑龙江中医药大学附属第二医院,哈尔滨150040 [3]黑龙江中医药大学附属第一医院妇科二科,哈尔滨150040

出　　处：《中华中医药杂志》2021年第3期1702-1705,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：黑龙江省自然科学基金面上项目(No.H2015026);黑龙江省中医药管理局项目(No.ZHY18-087)。

摘　　要：目的:探讨膈下逐瘀汤加减方治疗气滞血瘀型输卵管炎的可能作用机制。方法:60只大鼠随机分为正常组,模型对照组,中药高、中、低浓度组,每组12只。除正常组外,其余4组建立气滞血瘀型输卵管炎大鼠模型;中药高、中、低浓度组分别给予高、中、低剂量膈下逐瘀汤加减方混悬液;模型对照组灌服等体积蒸馏水。在末次给药后取材,肉眼观察输卵管形态变化,显微镜下观察输卵管病理学变化,并对大鼠外周血进行血流变学相关指标检测,Elisa法检测IL-4、IL-8的含量。结果:中药各浓度组较模型对照组能够不同程度改善血液流变学的部分指标(P<0.01,P<0.05)及输卵管的病变程度;且均能改善IL-4、IL-8水平(P<0.05),其中中药高浓度组IL-4、IL-8含量显著高于中药中、低浓度组(P<0.05)。结论:膈下逐瘀汤加减方可能通过调控促炎因子/抑炎因子平衡,调节机体免疫失调,达到抑制炎症反应的目的,进而治疗输卵管炎。Objective: To explore the possible mechanism of treatment salpingitis with qi stagnation and blood stasis type by modified Gexia Zhuyu Decoction(GD). Methods: Sixty rats were randomly divided into normal group, model control group and Chinese medicine high, medium and low dose groups, with 12 rats in each group. In addition to the normal group, the other four groups established the rat model of qi stagnation and blood stasis type of salpingitis. The high, medium and low dosage groups were given high, medium and low doses of GD suspension, respectively, the model control group was given equal volume distilled water. After the last administration, the material was collected, the morphological changes of the fallopian tube were observed by naked eyes, pathological changes of the fallopian tube were observed under a microscope, and hemorheology related indexes were detected in peripheral blood of rats, and the contents of IL-4 and IL-8 were detected by Elisa. Results: Compared with the model control group, Chinese medicine high, medium and low dose groups could improve some indicators of hemorheology(P<0.01, P<0.05) and the degree of tubal lesions in different degrees. Chinese medicine high, medium and low dose groups all improved the levels of IL-4 and IL-8(P<0.05), among which the effect of the high dose group was significantly better than that of the medium dose group and low dose group(P<0.05). Conclusion: Modified GD may regulate the balance of pro-inflammatory factors/anti-inflammatory factors, regulate immune disorders, and thus achieve the purpose of inhibiting inflammatory response for treating salpingitis.

关 键 词：膈下逐瘀汤加减方 气滞血瘀 输卵管炎 白介素-4 白介素-8 机制 免疫 血流变 

分 类 号：R285.5[医药卫生—中药学]