阳明病(阳明腑实证)模型大鼠“欲解时”相关炎症因子变化的研究  

Study on changes of inflammatory factors of the rats with Yangming disease syndrome of excess of Yangming fu-viscera in ‘recovery of disease’

作　　者：李冰鹄 张广梅[1] 赵协慧[1] 王仁嫒[1] 杨艳[1] 陈湘宏[1] 刘占厚[1] 吴萍[1] LI Bing-hu;ZHANG Guang-mei;ZHAO Xie-hui;WANG Ren-ai;YANG Yan;CHEN Xiang-hong;LIU Zhan-hou;WU Ping(Medical College of Qinghai University,Xining 810001,China)

机构地区：[1]青海大学医学院,西宁810001

出　　处：《中华中医药杂志》2021年第3期1740-1743,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金地区科学基金项目(No.81760825)。

摘　　要：目的:通过观察阳明病(阳明腑实证)模型大鼠"欲解时"血常规及肠黏膜上有关炎症因子的变化,初步探讨阳明病"欲解时"的病理生理学基础。方法:实验大鼠随机分为欲解时对照组、欲解时模型组、非欲解时对照组、非欲解时模型组,建立阳明腑实证大鼠模型后,分别于阳明病"欲解时"及"非欲解时"每天取血及肠组织,连续5d,血细胞分析仪检测血常规,免疫组化法测Toll样受体4(TLR4)蛋白含量,荧光实时定量聚合酶链反应(PCR)检测肠组织TLR4 mRNA水平。结果:欲解时模型组大鼠白细胞、中性粒细胞、单核细胞数较非欲解时模型组升高,其中单核细胞数上升差异有统计学意义(P<0.05);模型组淋巴细胞显著低于对照组(P<0.05)。欲解时模型组TLR4 mRNA及蛋白较非欲解时模型组显著降低,第1天TLR4 mRNA相对表达量差异有统计学意义(P<0.05)。结论:阳明腑实证病程中,欲解时模型组大鼠中性粒及淋巴细胞较对照组体现出较早较长时间的差异性,意味着欲解时的免疫反应更为明显。通过TLR4检测表明欲解时阳明腑实证的炎症反应较非欲解时轻,可能与其表达下调相关炎性介质的释放减少有关。Objective: By observing the changes of blood routine and related inflammatory factors on the intestinal mucosa of Yangming disease(syndrome of excess of Yangming fu-viscera) model rats ’recovery of disease’, preliminary explore the pathophysiological basis of Yangming disease in ’recovery of disease’. Methods: The experimental rats were randomly divided into the ’recovery of disease’ control group, ’recovery of disease’ model group, the ’non recovery of disease’ control group and the ’non recovery of disease’ model group. After establishing the Yangming diease model, blood and intestinal tissues were taken every day for 5 consecutive days, blood routine was detected by blood cell analyzer, TLR4 protein content was measured by immunohistochemistry, real-time quantitative aggregation by fluorescence the level of TLR4 mRNA was detected by PCR. Results: The number of leukocytes, neutrophils and monocytes was higher in the Yujie Time model group compared to the NonYujie Time model group, with a significant difference in the number of monocytes(P<0.05);the number of lymphocytes in the model group was significantly lower than that in the control group(P<0.05). TLR4 mRNA expression and protein content were significantly lower in the Yujie Time model group compared with the Non-Yujie Time model group, and there was a significant difference in the relative expression of TLR4 mRNA on day 1(P<0.05). Conclusion: Neutrophils and lymphocytes in the rats in the Yujie Time model group showed earlier and longer differences than those in the control group during the course of the Yang Ming internal organs evidence, implying a more pronounced immune response in Yujie Time. The TLR4 assay showed that the inflammatory response in the Yangming internal organs of Yujie Time is less severe than that of Non-Yujie Time, which may be related to the reduced release of inflammatory mediators associated with the down-regulation of its expression.

关 键 词：阳明腑实证 欲解时 肠源性内毒素血症 脂多糖 Toll样受体4 机制 炎症因子 

分 类 号：R255[医药卫生—中医内科学] R-332[医药卫生—中医学]