Single-cell transcriptomic landscape of human blood cells  

作　　者：Xiaowei Xie Mengyao Liu Yawen Zhang Bingrui Wang Caiying Zhu Chenchen Wang Qing Li Yingying Huo Jiaojiao Guo Changlu Xu Linping Hu Aiming Pang Shihui Ma Una Wang Wenbin Cao Shulian Chen Qiuling Li Sudong Zhang Xueying Zhao Wen Zhou Hongbo Luo Guoguang Zheng Erlie Jiang Sizhou Feng Lixiang Chen Lihong Shi Hui Cheng Sha Hao Ping Zhu Tao Cheng 

机构地区：[1]State Key Laboratory of Experimental Hematology and National Clinical Research Center for Blood Diseases,Institute of Hematology and Blood Diseases Hospital,Chinese Academy of Medical Sciences&Peking Union Medical College,Tianjin 300020,China [2]Center for Stem Cell Medicine and Department of Stem Cell&Regenerative Medicine,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300020,China [3]Cancer Research Institute,School of Basic Medical Science,Central South University,Changsha 410078,China [4]School of Life Sciences,Zhengzhou University,Zhengzhou 450001,China

出　　处：《National Science Review》2021年第3期37-47,共11页国家科学评论（英文版）

基　　金：supported by the Ministry of Science and Technology of China(2018YFA0107801,2016YFA0100600,2017YFA0103400);the National Key Research and Development Program of China(2018YFA0107804);the National Natural Science Foundation of China(81670106,81421002,81730006,81890990,81970104,32000803);the CAMS Initiative for Innovative Medicine(2017-I2M-1-015,2019-I2M-1-006,2016-I2M-1-017,2019-I2M-2-001)。

摘　　要：High throughput single-cell RNA-seq has been successfully implemented to dissect the cellular and molecular features underlying hematopoiesis.However,an elaborate and comprehensive transcriptome reference of the whole blood system is lacking.Here,we profiled the transcriptomes of 7551 human blood cells representing 32 immunophenotypic cell types,including hematopoietic stem cells,progenitors and mature blood cells derived from 21 healthy donors.With high sequencing depth and coverage,we constructed a single-cell transcriptional atlas of blood cells(ABC) on the basis of both protein-coding genes and long noncoding RNAs(lncRNAs),and showed a high consistence between them.Notably,putative lncRNAs and transcription factors regulating hematopoietic cell differentiation were identified.While common transcription factor regulatory networks were activated in neutrophils and monocytes,lymphoid cells dramatically changed their regulatory networks during differentiation.Furthermore,we showed a subset of nucleated erythrocytes actively expressing immune signals,suggesting the existence of erythroid precursors with immune functions.Finally,a web portal offering transcriptome browsing and blood cell type prediction has been established.Thus,our work provides a transcriptional map of human blood cells at single-cell resolution,thereby offering a comprehensive reference for the exploration of physiological and pathological hematopoiesis.

关 键 词：human hematopoietic cells lnc RNAs transcription factor single-cell RNA-seq 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]