铜掺入型钛合金对破骨细胞分化影响的体外研究  

Copper-doped Titanium Alloy Inhibited RANKL-induced Osteoclasts Differentiation in Vitro

作　　者：许雄程 卓瑾 徐燕梅 骆凯[1,2] 陈超[2] 钟泉[2] XU Xiongcheng;ZHUO Jin;XU Yanmei;LUO Kai;CHEN Chao;ZHONG Quan(Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China;Institute of Stomatology & Laboratory of Oral Tissue Engineering, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China)

机构地区：[1]福建省口腔疾病研究重点实验室,福建省口腔生物材料工程技术研究中心,福建省高校口腔医学重点实验室,福建医科大学口腔医学院/附属口腔医院,福建福州350002 [2]福建医科大学口腔医学研究院,福建医科大学口腔组织工程研究中心,福建医科大学口腔医学院/附属口腔医院,福建福州350002

出　　处：《口腔医学研究》2021年第4期371-374,共4页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81870766);福建省自然科学基金项目(编号:2017J01805);福建省发改委第三批省级预算内投资计划项目;福建医科大学启航基金项目(编号:2019QH2041)。

摘　　要：目的:研究铜掺入型钛合金对破骨细胞分化的影响。方法:通过激光熔化技术制备掺铜的钛合金。使用RANKL诱导小鼠单核细胞分化,制备破骨细胞。将掺铜的钛合金与破骨细胞共培养,通过观察细胞形态、记录破骨细胞数目以及破骨相关基因的表达,分析掺铜的钛合金对破骨细胞分化相关基因表达的影响。结果:小鼠单核细胞与RANKL共培养后可分化成破骨细胞。掺铜的钛合金能够抑制单核细胞向破骨细胞分化,同时还能降低破骨分化相关基因CTSK、TRAP、NFATc1和NF-κB的表达。结论:掺铜的钛合金可抑制破骨细胞的活性,有望应用于骨再生手术中个性化钛网的制备。Objective:To study the effect of copper-doped titanium alloys on osteoclasts differentiation.Methods:Copper-doped titanium alloys were prepared by using the technique of selective laser melting(SLM).Mouse monocytes(RAW264.7)were induced to differentiate to osteoclasts by RANKL.Copper-doped titanium alloys was co-cultured with osteoclasts.The morphology of osteoclasts was observed under a fluorescence microscope.The number of osteoclasts was recorded,and the expression of genes related to osteoclast differentiation was detected by real-time fluorescence quantitative PCR.Results:Induced by RANKL,mouse monocytes could differentiate into osteoclasts in vitro.Copper-doped titanium alloys could not only inhibit the differentiation of monocytes(RAW264.7)which generated osteoclasts,but also inhibit the expression of osteoclast-related genes CTSK,TRAP,NFATc1,and NF-κB.Conclusion:Copper-doped titanium alloys can inhibit the activity of osteoclasts and is expected to be used in the preparation of titanium mesh which has potential application value for alveolar bone regeneration.

关 键 词：钛合金 铜 破骨分化 支架材料 

分 类 号：TG1[金属学及工艺—金属学]