广金钱草中超声波辅助提取总黄酮的工艺优化  

Optimization of ultrasonic assisted extraction of total flavonoids from Herba Lysimachia Christina

作　　者：蔡伟[1] 李敏 施亚宁 李兆慧[1] 陈梦婷 何欣 罗益远 CAI Wei;LI Min;SHI Ya-ning;LI Zhao-hui;CHEN Meng-ting;HE Xin;LUO Yi-yuan(College of Traditional Chinese Medicine,Zhejiang Pharmaceutical College,Ningbo,351100,China;College of Traditional Chinese Medicine,JiLin Agricultural Science and Technology University,Jilin 132101,China)

机构地区：[1]浙江医药高等专科学校中药学院,宁波315100 [2]吉林农业科技学院中药学院,吉林132101

出　　处：《人参研究》2021年第2期27-31,共5页Ginseng Research

基　　金：浙江省重点建设高校优势特色学科开放基金项目,项目编号:ZYAOX2018028;宁波市自然科学基金项目,项目编号:2018A610434;浙江医药高等专科学校校课题,项目编号:2019013;浙江省大学生新苗人才计划,项目编号:2018R458003,2020R424003。

摘　　要：利用纤维素酶协同超声波提取技术对广金钱草中总黄酮的提取工艺进行筛选,通过单因素试验和L_(9)(3^(4))正交试验优选出纤维素酶协同超声波法提取总黄酮的最佳工艺为:乙醇浓度50%、料液比1:30,纤维素酶500mg,超声波提取时间30min。在该提取条件下黄酮得率为2.28%。本实验优选的工艺设计合理,稳定可行,抗氧化活性较好,为鱼腥草的进一步研究提供技术基础。The extraction process of total flavonoids from Herba Lysimachia Christina were screened by using cellulase synergistic ultrasonic extraction technology.With rutin as the reference substance,the extraction rate of flavonoids was the index.The optimum conditions for the extraction of total flavonoids by cellulase and ultrasonic method were optimized by single factor test and L_(9)(3^(4))orthogonal test.The ultrasonic treatment time is 30min,ethanol volume fraction is 50%,rate of material and solution 1:30,500mg cellulose.The extraction rate of flavonoids under this process condition was 2.28%.The optimized extraction process was scientific,stable and feasible,can provided a new basis for the comprehensive assessment and overall control of the quality of Herba Lysimachia Christina.

关 键 词：广金钱草 正交试验 黄酮 超声波辅助提取 

分 类 号：R284.2[医药卫生—中药学]