儿童体重指数与肠道菌群多样性的关联性研究  

Association study between body mass index and gut microbial diversity among children

作　　者：古灼和[1] 陈赞雄 董光辉[2] 曾晓雯[2] 谢彦奇[1] 李青青 Gu Zhuohe;Chen Zanxiong;Dong Guanghui;Zeng Xiaowen;Xie Yanqi;Li Qingqing(Department of Nutriology,Maternal and Children’s Health Hospital of Maoming,Guangdong,Maoming 525000,China;Guangzhou Key Laboratory of Environmental Pollution and Health Risk Assessment,Department of Occupational and Environmental Health,School of Public Health,Sun Yat-sen University,Guangdong,Guangzhou 510080,China)

机构地区：[1]茂名市妇幼保健院营养科,广东茂名525000 [2]中山大学公共卫生学院劳动卫生与环境卫生学系,广州市环境污染与健康风险评价重点实验室,广东广州510080

出　　处：《发育医学电子杂志》2021年第2期103-108,共6页Journal of Developmental Medicine (Electronic Version)

基　　金：广东省科技计划项目(2017B020246033)。

摘　　要：目的对儿童体重指数(body mass index,BMI)与肠道菌群多样性的关联性进行初步探讨。方法采用横断面调查方法,从2018年7月1日至7日在广东省茂名市共招募87名儿童,平均年龄(11±2)岁。以世界卫生组织2006年发布的儿童体格生长指标百分位数参考值为标准,将研究对象分成偏瘦、正常、超重以及肥胖4个组。通过Illumina Miseq高通量测序方法,对粪便样本细菌16S V4~V5区进行碱基测序;采用广义线性模型分析儿童BMI与Chao、Ace、Shannon以及Simpson指数等肠道菌群Alpha多样性指标的相关性。结果BMI每升高1 kg/m^(2),肥胖儿童的Shannon指数降低1.31%(95%CI:-2.31%~-0.25%),Simpson指数上升3.89%(95%CI:1.01%~6.83%);而偏瘦儿童的Ace指数下降4.27%(95%CI:-8.14%~-0.24%)。此外,趋势性检验结果显示,随BMI四分位数的增加,Shannon指数呈下降趋势(P=0.042)。结论儿童BMI与肠道菌群多样性存在关联,且随着BMI的增加,肠道菌群多样性呈下降趋势。此外,不同体型儿童中BMI与肠道菌群的关联强度可能不同。Objective To explore the association between body mass index(BMI)and gut microbial diversity among children.Methods A cross-sectional study was conducted,87 children with average age(11±2)years were recruited from 1st to 7th July 2018 in Maoming,Guangdong province.According to the child growth reference reported by WHO in 2006,the subjects were separated into four groups:lean,normal,overweight and obesity group.We detected the base sequences in 16S V4-V5 region of gut flora from fecal samples by Illumina Miseq high-throughput method,and then we performed generalized linear models to assess the relations of BMI and the indexs of gut microbial diversity including of Chao,Ace,Shannon and Simpson index among these children.Results With per 1 kg/m2 increment of BMI in obesity children,Shannon index decreased by 1.31%(95%CI:-2.31%,-0.25%),Simpson index inversely increased by 3.89%(95%CI:1.01%,6.83%).As for lean children,Ace index had the decrement of 4.27%(95%CI:-8.14%,-0.24%).Additionally,trend analysis showed that Shannon index(P=0.042)tend to be decreased with the increase of BMI quartiles.Conclusions BMI is related with gut microbial diversity among children,gut microbial diversity tended to be descended along with increased BMI.Moreover,aforementioned relations may vary by groups.

关 键 词：肠道菌群多样性 体重指数 儿童 肥胖 

分 类 号：R723.14[医药卫生—儿科]