Optimization of the Ultrasonic-assisted Extraction Process for Total Flavonoids in Primula sikkimensis Hook.by Response Surface Methodology  

作　　者：Qing XIA Dandan TANG Zhiqiang ZHANG Jianhui JIANG 

机构地区：[1]Sichuan College of Traditional Chinese Medicine,Mianyang 621000,China

出　　处：《Medicinal Plant》2021年第1期35-39,共5页药用植物：英文版

基　　金：Supported by Project of Sichuan College of Traditional Chinese Medicine(18ZRYB04).

摘　　要：[Objectives]To optimize the ultrasonic-assisted extraction process for total flavonoids in Primula sikkimensis Hook.[Methods]The effects of ethanol volume fraction,extraction time,extraction temperature,ultrasonic time and solid-to-liquid ratio on the yield of total flavonoids were investigated by single factor method,and the extraction process for total flavonoids in P.sikkimensis was optimized by Box-Behnken experimental design.[Results]The optimal extraction process for total flavonoids in P.sikkimensis was ethanol volume fraction 45%,extraction time 29 min,extraction temperature 80℃,solid-to-liquid 1∶40(g/mL)and ultrasonic time 10 min.Under such conditions,the average yield of total flavonoids in P.sikkimensis was 3.09%.[Conclusions]The optimized extraction process is simple and feasible,and is expected to provide a reference for the extraction of total flavonoids in P.sikkimensis.

关 键 词：Primula sikkimensis Hook. Total flavonoids Response surface methodology(RSM) Extraction process 

分 类 号：R284.1[医药卫生—中药学]