Abp1在调节性T细胞的发育及功能中的作用  

Roles of Abp1 in the development and function of regulatory T cells

作　　者：阮昌顺 杨迪 杜作晨 李晗 胡乐玲 周丽佳 简鼎 黄璐[1] RUAN Changshun;YANG Di;DU Zuochen;LI Han;HU Leling;ZHOU Lijia;JIAN Ding;HUANG Lu(Department of Pediatric Research Institute Children's Hospital of Chongqing Medical University,National Clinical Research Center for Child Health and Disorders,Ministry of Education Key Laboratory of Child Development and Disorders,Chongqing Key Laboratory of Child,Infection and Immunity,China International Science and Technology Cooperation Base of Child Development and Critical Disorders,Children9 s Hospital of Chongqing Medical University,Chongqing 400014,China)

机构地区：[1]重庆医科大学附属儿童医院儿研所国家儿童健康与疾病临床医学研究中心儿童发育疾病研究教育部重点实验室儿童感染免疫重庆市重点实验室儿童发育重大疾病国家国际科技合作基地,400014

出　　处：《免疫学杂志》2021年第4期289-299,共11页Immunological Journal

基　　金：重庆市科学技术委员会科研项目(cstc2017jcyjAX0439);美捷登基金(MJR20180012)。

摘　　要：目的探讨肌动蛋白连接蛋白1(actin-binding protein 1,Abp1)对调节性T细胞(Treg)发育及功能的影响。方法通过流式细胞术检测野生型小鼠(WT组)和Abp1基因敲除小鼠(KO组)的胸腺、脾脏、浅表淋巴结及肠系膜淋巴结中T淋巴细胞及Treg细胞的比例、数量以及相关功能性分子的表达,并利用体外抑制试验检测Abp1缺失对Treg抑制功能的影响。结果与WT小鼠相比,Abp1 KO小鼠的胸腺、脾脏、浅表淋巴结及肠系膜淋巴结中的Treg的比例和细胞数目均没有明显的变化,同时缺失Abp1的Treg细胞的主要功能性分子ICOS、CTLA4、PD-1和NRP-1的表达亦没有显著的差异,进一步实验发现缺失Abp1的Treg细胞对初始T细胞向活化T细胞分化的抑制作用较WT相比无明显变化。结论Abp1对Treg细胞的发育和功能没有显著影响。This study was designed to investigate the effect of progranulin actin-binding protein 1(Abp1)on the differentiation and function of regulatory T cell,and the molecule mechanism.We detected the proportion,number and functional molecule expression of T lymphocytes and Treg cells in the thymus,spleen,peripheral lymph nodes and mesenteric lymph nodes of wild-type mice(WT group)and Abp1 knockout mice(KO group)by cytometry flow,and the effect of Abp1 deletion on the inhibitory function of Treg in vitro was measured as well.Compared with WT mice,Abp1 KO mice had no significant differences on the proportion and number of Tregs in the thymus,spleen,peripheral lymph nodes and mesenteric lymph nodes,meanwhile,the expression of the main functional molecules ICOS,CTLA4,PD-1 and NRP-1 in Treg cells lacking Abp1 were not significantly different.Furthermore,Abp1-absent Treg cells showed no significant changes in inhibiting T cells activation as comparing with WT Treg cells.In conclusion,Abp1 has no significant effect on the development and function of Treg cells.

关 键 词：肌动蛋白连接蛋白1 调节性T细胞 抑制功能 

分 类 号：R392[医药卫生—免疫学]