利用玉米-大刍草渗入系群体解析玉米茎长和茎粗的遗传基础  

Dissecting the Genetic Basis of Stem Length and Stem Diameter in Maize(Zea mays)Using a Maize-teosinte Introgression Line Population

作　　者：李芳 柳志华 胡锦祥 肖仁杰 朱雪晴 徐莹 邓敏 李瑞莲[1,2] 罗红兵 黄成[1,2,3] LI Fang;LIU Zhi-Hua;HU Jin-Xiang;XIAO Ren-Jie;ZHU Xue-Qing;XU Ying;DENG Min;LI Rui-Lian;LUO Hong-Bing;HUANG Cheng(College of Agronomy,Hunan Agricultural University,Changsha 410128,China;Maize Engineering Technology Research Center of Hunan Province,Changsha 410128,China;Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop,Changsha 410128,China)

机构地区：[1]湖南农业大学农学院,长沙410128 [2]湖南省玉米工程技术研究中心,长沙410128 [3]湖南省作物种质创新与资源利用重点实验室,长沙410128

出　　处：《农业生物技术学报》2021年第2期216-223,共8页Journal of Agricultural Biotechnology

基　　金：湖南省自然科学基金(2020JJ5235);湖南省教育厅优秀青年项目(18B117);作物种质创新与资源利用重点实验室开放研究项目(19KFXM10);湖南农业大学“双一流”学科建设项目(SYL2019004)。

摘　　要：茎长和茎粗作为玉米(Zea mays)茎秆重要的两个性状,不仅与玉米抗倒伏性密切相关,而且直接或间接影响玉米产量和株型,是玉米育种的重要目标性状。本研究利用玉米自交系W22与玉米野生祖先种大刍草(Z.mays ssp.parviglumis)杂交衍生得到的包含866个家系的渗入系群体,结合19838个SNP分子标记,采用R/qtl的多QTL模型对玉米茎长和茎粗进行高精度的QTL定位分析。结果表明,玉米茎长和茎粗均存在广泛的遗传变异,属于典型的数量性状,由微效多基因控制。共检测到2个控制玉米茎长的QTLs,分别位于第1和4染色体,表型贡献率分别为4.51%和2.68%,加性效应分别为0.41 cm和0.39cm。共检测到5个控制玉米茎粗的QTLs,分别位于第1、2、5、6和7染色体,单个茎粗QTL表型贡献率的变幅为2.06%~8.32%,加性效应的变幅为0.03~0.09 cm。进一步对本研究定位到的最大效应的茎粗QTL(q SD5-1)进行候选基因分析,筛选出13个可能的候选基因。本研究为进一步解析玉米茎长和茎粗的遗传基础、克隆相关基因和分子标记辅助选择育种提供参考依据。Stem length and stem diameter,as two important traits of maize(Zea mays)stem,are closely related to maize lodging resistance,also directly or indirectly affect maize yield and plant architecture,and they are important target traits in maize breeding.This study performed a high-resolution QTL mapping for stem length and stem diameter using the multiple QTL model implemented in R/qtl,together with 19838 SNP markers.The introgression line population of 866 lines was derived from a cross between a typical maize inbred line W22 and a typical accession of teosinte.The results showed that there were extensive genetic variations in stem length and stem diameter,which were typical quantitative traits controlled by multiple micro-effect genes.A total of 2 QTLs controlling stem length was located on chromosome 1 and 4 respectively,with the phenotypic contribution rate of 4.51%and 2.68%,and the additive effect of 0.41 and 0.39 cm,respectively.In addition,5 QTLs controlling stem diameter were located on chromosome 1,2,5,6 and 7,respectively,and the phenotypic contribution rate of each QTL varied from 2.06%to 8.32%,and the additive effect varied from 0.03 to 0.09 cm.Further analysis of candidate genes for the largest effect QTL(q SD5-1)of stem diameter,13 possible candidate genes were screened out.This study might provide a theoretical basis for elucidating the genetic basis of stem length and stem diameter,the cloning of related genes and molecular marker-assisted selection breeding in maize.

关 键 词：玉米 大刍草 茎长 茎粗 遗传基础 

分 类 号：S513[农业科学—作物学]