红麻转录因子基因HcNAC1的分离及其在干旱胁迫下的表达模式  

Isolation of HcNAC1 Transcription Factor Gene and Its Expression Patterns Under Drought Stress in Kenaf(Hibiscus cannabinus)

作　　者：邓接楼[1] 张高阳[1] 张宗良[1] 张超 徐建堂 祁建民 伍应保 王刚 DENG Jie-Lou;ZHANG Gao-Yang;ZHANG Zong-Liang;ZHANG Chao;XU Jian-Tang;QI Jian-Min;WU Ying-Bao;WANG Gang(College of Life Sciences,Shangrao Normal University,Shangrao 334001,China;College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]上饶师范学院生命科学学院,上饶334001 [2]福建农林大学农学院,福州350002

出　　处：《农业生物技术学报》2021年第2期251-257,共7页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31860396);江西省教育厅科学技术研究项目(K6000213)。

摘　　要：干旱是严重影响农作物生长的非生物限制因子之一,NAC (NAM, ATAF1/2和CUC2)转录因子是植物类特有的一类转录因子,在调控植物生长发育和应对非生物胁迫过程中发挥重要作用。为研究红麻(Hibiscus cannabinus)中NAC转录因子在干旱胁迫方面的调控功能,本研究从红麻转录组数据库中分离得到一个红麻NAC类基因,命名为HcNAC1 (Gen Bank No. MT799841)。序列分析发现HcNAC1基因编码区包含1 044 bp,编码347个氨基酸,蛋白质分子量38.77 k D,等电点为8.78。HcNAC1具有NAM/NAC类基因家族的保守结构域,在该蛋白的N端含有160个氨基酸组成的NAC结构域,具有两个核定位信号(nuclear localization signal, NLS)序列。系统发育进化树分析表明红麻HcNAC1基因与木槿(Hibiscus syriacus)的进化关系最近。利用反转录PCR (reverse transcription PCR, RT-PCR)和q RT-PCR对该基因在不同组织部位和干旱胁迫处理24 h内的表达量进行了检测,结果表明:HcNAC1在根、茎、叶、花和果实中均有表达,叶片组织中HcNAC1随着聚乙二醇6000 (PEG6000)干旱处理的时间的延长,其表达量也逐步增加,处理12 h后,表达量达到最大值,随后逐渐下降,说明HcNAC1在响应干旱胁迫中发挥着重要的调控作用。本研究探究了HcNAC1基因表达与干旱胁迫的相互关系,为后期研究红麻NAC转录因子参与干旱逆境胁迫的分子机制提供参考依据。Drought is one of the abiotic limiting factors that seriously affect the growth of crops. NAC(NAM, ATAF and CUC) transcription factor is one type of transcription factors in plants, it play an important role in regulating plant growth, development and response to abiotic stress. In order to study the regulation function of NAC transcription factor in drought stress of kenaf(Hibiscus cannabinus), a NAC like gene was isolated from kenaf transcriptome database, named HcNAC1 gene(Gen Bank No. MT799841). The coding sequence of HcNAC1 contained 1 044 bp, encoding 347 amino acids, protein molecular weight 38.77 k D,isoelectric point was 8.78. The HcNAC1 genes with NAM/NAC family conservative area, containing about 160 amino acid conservative in the protein N-terminal structural domain, with 2 nuclear localization signal(NLS) sequence. Phylogenetic tree analysis showed that kenaf HcNAC1 had the closest evolutionary relationship with Hibiscus syriacus. The expression level in different tissues and within 24 h of drought stress was detected by reverse transcription PCR(RT-PCR) and q RT-PCR, research results showed that HcNAC1 gene was expressed in root, stem, leaf, flower and fruit, HcNAC1 gene was expressed under PEG6000 stress and had most expression level at 12 h, and then the expression level gradually decreased. HcNAC1 played an important regulatory role in response to drought stress. The study explored the correlation between HcNAC1 expression and drought stress. and could provide a reference for further research on the molecular mechanism of HcNAC1 transcription factors involved in drought stress of kenaf.

关 键 词：红麻 NAC转录因子 干旱胁迫 表达模式分析 

分 类 号：S563.5[农业科学—作物学]