枯草芽胞杆菌基因组删减对异源酶表达影响的研究进展  

Advance in the effect of reduction of the Bacillus subtilis genome on the expression of heterologous enzymes

作　　者：张金方 李昕悦 徐小健 陈雪佳 牛馨 任绍东 李玉 路福平 ZHANG Jinfang;LI Xinyue;XU Xiaojian;CHEN Xuejia;NIU Xin;REN Shaodong;LI Yu;LU Fuping(Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院工业发酵微生物教育部重点实验室,天津300457

出　　处：《微生物学通报》2021年第3期859-872,共14页Microbiology China

基　　金：国家重点研发计划(2017YFB0308401)。

摘　　要：枯草芽胞杆菌作为一种遗传背景清晰、基因编辑成熟的革兰氏阳性菌,是多种重要工业酶的生产宿主。随着转录组、蛋白质组、代谢组等多组学测序和分析技术的发展,通过合理设计简化枯草芽胞杆菌基因组,减少细胞内冗余的调控和代谢网络,使得细胞更精简且便于控制,展现出了枯草芽胞杆菌作为异源酶表达宿主细胞的应用潜力。本文简要综述了枯草芽胞杆菌基因组删减的研究进展,归纳了必需基因的确定方法,重点介绍了枯草芽胞杆菌通过删减基因组提升异源酶表达的研究进展及删减策略,充分展示了枯草芽胞杆菌基因组删减在构建异源酶表达底盘细胞中的重要作用。Bacillus subtilis as a Gram-positive bacterium,which has clear genetic background and mature gene editing,and it was also considered as the important host of industrial enzymes.With the development of multi-omics sequencing technologies including transcriptome,proteome and metabolome,the rational design could simplify the Bacillus subtilis genome,reduce redundant regulatory and metabolic networks.It makes Bacillus subtilis more streamlined and easily to control.Furthermore,Bacillus subtilis as a heterologous enzyme expression host has been emerged the potential applications.This article briefly reviewed the advance in the effect Bacillus subtilis genome reduction,summarized the essential genes and the methods to identify them in Bacillus subtilis,highlighted the research progress and deletion strategies of Bacillus subtilis to enhance heterologous enzymes by deleting the genome,and demonstrated the important role of genome reduction in the construction of heterologous enzyme expression on the cellular chassis.

关 键 词：枯草芽胞杆菌 最小基因组 必需基因 基因组删减 异源酶表达 

分 类 号：Q78[生物学—分子生物学]