Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase Ⅱ oocytes  

作　　者：Ying Huo Peng Yuan Qingyuan Qin Zhiqiang Yan Liying Yan Ping Liu Rong Li Jie Yan Jie Qiao 

机构地区：[1]Center for Reproductive Medicine,Department of Obstetrics and Gynecology,Peking University Third Hospital,Beijing 100191,China [2]Department of Physiology and Pathophysiology,School of Basic Medical Sciences,Peking University,Beijing 100191,China [3]Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction,Beijing 100191,China [4]Key Laboratory of Assisted Reproduction,Ministry of Education,Beijing 100191,China [5]Peking-Tsinghua Center for Life Sciences,Peking University,Beijing 100871,China [6]National Clinical Research Center of Obstetrics and Gynecology,Beijing 100191,China

出　　处：《Frontiers of Medicine》2021年第1期144-154,共11页医学前沿（英文版）

基　　金：supported by grants from the National Key Research and Development Program(Nos.2017YFC1002000,2018YFC1004001,and 2016YFC1000600);the National Natural Science Foundation of China(Nos.81571386 and 31429004);the Capital Health Development Research Project(No.2018-2-4095).

摘　　要：Oocyte cryopreservation is widely used for clinical and social reasons.Previous studies have demonstrated that conventional slow-freezing cryopreservation procedures,but not storage time,can alter the gene expression profiles of frozen oocytes.Whether vitrification procedures and the related frozen storage durations have any effects on the transcriptomes of human metaphase Ⅱ oocytes remain unknown.Four women(30–32 years old)who had undergone IVF treatment were recruited for this study.RNA-Seq profiles of 3 fresh oocytes and 13 surviving vitrified-thawed oocytes(3,3,4,and 3 oocytes were cryostored for 1,2,3,and 12 months)were analyzed at a single-cell resolution.A total of 1987 genes were differentially expressed in the 13 vitrifiedthawed oocytes.However,no differentially expressed genes were found between any two groups among the 1-,2-,3-,and 12-month storage groups.Further analysis revealed that the aberrant genes in the vitrified oocytes were closely related to oogenesis and development.Our findings indicated that the effects of vitrification on the transcriptomes of mature human oocytes are induced by the procedure itself,suggesting that long-term cryostorage of human oocytes is safe.

关 键 词：human metaphaseⅡoocyte VITRIFICATION cryostorage duration single-cell RNA-Seq lncRNA 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]