^(60)Coγ射线全身照射大鼠的小肠组织中辐射敏感脂质代谢物的筛选  

Screening of radiosensitive lipid metabolites in rat small intestine after total body irradiation with ^(60)Coγ-rays

作　　者：习聪 赵骅[1] 陆雪[1] 蔡恬静[1] 田梅[1] 刘青杰[1] Xi Cong;Zhao Hua;Lu Xue;Cai Tianjing;Tian Mei;Liu Qingjie(Key Laboratory of Radiological Protection and Nuclear Emergency,China CDC,National Institute for Radiological Protection,Chinese Center for Disease Control and Prevention,Beijing 100088,China)

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室,北京100088

出　　处：《中华放射医学与防护杂志》2021年第3期172-177,共6页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金(81573081)。

摘　　要：目的筛选大鼠的小肠组织中辐射敏感脂质代谢物并探索其代谢通路,为放射性肠损伤生物标志物研究提供科学依据。方法用60Coγ射线对大鼠进行全身照射,照射剂量分别为0、1、2、3、5、8 Gy,通过液相色谱-质谱联用(LC-MS)的靶向脂质组学方法,探究大鼠受照后小肠组织中脂质代谢物的变化。结果大鼠受照后3 d,筛选出小肠组织中15个辐射敏感脂质代谢物,其中4个代谢物浓度显著上调,11个代谢物浓度显著下调,差异有统计学意义(t=-6.395、5.998、5.836、-5.503、-5.449、-5.422、4.841、4.802、4.621、4.457、4.426、4.373、4.110、3.945、3.902,P<0.05;错误发现率<0.05),主要涉及鞘脂代谢、甘油磷脂代谢等代谢通路。4个磷脂酰丝氨酸(PS)随受照剂量的上升而显著上升,1个磷脂酸(PA)、2个鞘磷脂(SM)及4个脂肪酸(FA)随受照剂量的上升而显著下降,具有明显的剂量-效应关系(R2>0.80,P<0.05)。结论大鼠受到60Coγ射线全身照射后,小肠组织中脂质代谢物鞘脂代谢、甘油磷脂代谢等代谢通路的11个脂质代谢物具有良好的剂量-效应关系,可能成为放射性肠损伤生物标志物。Objective To screen radiosensitive lipid metabolites in rat small intestine and analyze their metabolic pathways,in order to provide scientific basis for radiation enteropathy biomarkers.Methods The total body irradiation of 60Coγrays was performed to rats with different doses of 0,1,2,3,5 and 8 Gy.The changes of lipids in small intestine were studied by targeted lipidomics method based on liquid chromatography coupled mass spectrometry(LC-MS).Results Fifteen lipids in small intestine were screened as radiosensitive metabolites at 3 d after irradiation,including 4 up-regulated lipids and 11 down-regulated lipids(t=-6.395,5.998,5.836,-5.503,-5.449,-5.422,4.841,4.802,4.621,4.457,4.426,4.373,4.110,3.945,3.902,P<0.05 and FDR<0.05).The metabolic pathways of sphingolipid,glycerophosphoplipid were significantly enriched.Four phosphatidyl serines(PS)increased while 1 phosphatidic acid(PA),2 sphingomyelins(SM)and 4 fatty acids(FA)decreased in a good dose-response manner(R2>0.80,P<0.05),which were more potential radiation enteropathy biomarkers.Conclusions Lipid metabolites in rat small intestine were significantly changed after the rat was total body irradiated with 60Coγ-rays.Eleven lipids with good dose-response relationship were more potential to be radiation enteropathy biomarkers.

关 键 词：电离辐射 肠损伤 脂质组学 代谢通路 

分 类 号：R818[医药卫生—放射医学]