南亚热带马尾松-红椎混交林及其纯林土壤细菌群落结构与功能  

Structure and function of soil bacterial communities in the monoculture and mixed plantation of Pinus massoniana and Castanopsis hystrix in southern subtropical China

作　　者：梁艳 明安刚[2,3] 何友均 罗应华[1] 谭玲[1] 覃林[1,5] LIANG Yan;MING An-gang;HE You-jun;LUO Ying-hua;TAN Ling;QIN Lin(Guangxi Key Laboratory of Forest Ecology and Conservation,College of Forestry,Guangxi University,Nanning 530004,China;Experiment Center of Tropical Forestry,Chinese Academy of Forestr,Pingxiang 532600,Guangxi,China;Guangxi Youyiguan Forest Ecosystem Research Station,Pingxiang 532600,Guangxi,China;Research Institute of Forestry Policy and Information,Chinese Academy of Forestry,Beijing 100091,China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangxi University,Nanning 530004,China)

机构地区：[1]广西大学林学院,广西森林生态与保育重点实验室,南宁530004 [2]中国林业科学研究院热带林业实验中心,广西凭祥532600 [3]广西友谊关森林生态系统定位观测研究站,广西凭祥532600 [4]中国林业科学研究院林业科技信息研究所,北京100091 [5]广西大学,亚热带农业生物资源保护与利用国家重点实验室,南宁530004

出　　处：《应用生态学报》2021年第3期878-886,共9页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(31560109)资助。

摘　　要：营建乡土阔叶树种人工纯林和针阔混交林是我国亚热带地区森林经营的发展趋势,但对人工纯林和针阔混交林土壤细菌群落结构及功能知之甚少。本研究以南亚热带乡土针叶树种马尾松、阔叶树种红椎人工纯林及二者的混交林为对象,运用细菌16S rRNA基因高通量测序技术和PICRUSt基因功能预测,分析了3种人工林不同土层(0~20、20~40和40~60 cm)土壤细菌群落的结构与功能。结果表明:混交林和马尾松林土壤细菌群落结构相似,但与红椎林差异显著,红椎林土壤细菌群落多样性、生物通路代谢功能和氮循环功能低于马尾松林和混交林;土壤全氮、硝态氮和C/N是导致红椎林与马尾松林和混交林土壤细菌群落结构及功能差异的主要土壤理化因子。就土壤细菌群落结构与功能而言,在该地区营造红椎和马尾松针阔混交林要优于红椎纯林。Establishing monoculture of native broadleaved tree species and mixed coniferous broadleaved plantations is the tendency for forest management in subtropical China. The variations of structure and function of soil bacterial community in monoculture and mixed tree plantations are still not clear. We examined soil bacterial community structure and function under different soil layers(0-20, 20-40 and 40-60 cm) in three planted forests, including broadleaved Castanopsis hystrix, coniferous Pinus massoniana and their mixed plantation, in south subtropical China, using 16 S rRNA gene high-throughput sequencing and PICRUSt prediction. The results showed that soil bacterial community structure of mixed plantation and P. massoniana plantation were similar but being significant different from that in C. hystrix plantation. The diversity, biological pathways metabolic function, and nitrogen cycling function of soil bacterial community in C. hystrix plantation were lower than those in P. massoniana plantation and mixed plantation. Soil total nitrogen, nitrate nitrogen and C/N were the main factors driving the variations of soil bacterial community structure and function among different forest types. Our results suggested that the mixed plantation of C. hystrix and P. massoniana is better than C. hystrix plantation in this area in terms of soil bacterial community structure and function.

关 键 词：土壤细菌群落 高通量测序技术 PICRUSt基因功能预测 氮循环功能基因 人工林 

分 类 号：S714.3[农业科学—林学]