重楼皂苷I在大鼠膝骨关节炎中的治疗作用  

Therapeutic effect of Papilioside I on knee osteoarthritis in rats

作　　者：孙星 李承祥 陈扬扬 皮国富[1] Sun Xing;Li Cheng-xiang;Chen Yang-yang;Pi Guo-fu(The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]郑州大学第一附属医院骨科,郑州450000

出　　处：《医药论坛杂志》2021年第4期32-35,共4页Journal of Medical Forum

摘　　要：目的探讨不同剂量重楼皂苷I(PPI)对木瓜蛋白酶诱导的骨关节炎(OA)大鼠的治疗作用。方法将30只同龄雄性SD大鼠随机分为3组,每组10只,分别为:模型组、低剂量PPI组和高剂量PPI组。木瓜蛋白酶注射于右侧膝关节腔以建立大鼠膝关节OA模型,7天(d)后开始灌胃,低剂量PPI组灌服溶解于DMSO的重楼皂苷I2 mg/(kg·d),高剂量PPI组灌服溶解于DMSO的重楼皂苷I5 mg/(kg·d),模型组灌服等量二甲基亚砜(DMSO)。连续灌胃3周后通过大鼠眼眶静脉丛收集静脉血,离心后测定血清中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)及肿瘤坏死因子(TNF-α)三种细胞因子表达水平;颈椎脱臼法处死大鼠后取膝关节标本固定脱钙后制作冰冻切片,并进行HE染色、番红O染色以及Mankin评分分析。结果 PPI显著下调大鼠血液中TNF-α、IL-1β、IL-6的表达。高剂量与低剂量的PPI干预对大鼠血清三种细胞因子的下调率与模型组比较,组间差异有统计学意义(P<0.05);OA模型组膝关节软骨表面粗糙、软骨下骨硬化、骨赘增生、裂隙形成、严重滑膜炎症;PPI干预组软骨层损伤修复显著,表面相对光滑,滑膜炎症表现明显改善,未见明显增生骨赘。模型组与低剂量PPI组及高剂量PPI组Mankin评分差异有统计学意义(P<0.05),高剂量PPI组与低剂量PPI组组间差异有统计学意义(P<0.05)。结论重楼皂苷I能够改善和修复受损退变的关节软骨,具有降低关节内炎症的作用。本研究为重楼皂苷I成为治疗膝关节骨性关节炎临床药物的潜在可行性提供了实验依据支持。Objective To investigate the therapeutic effect of different doses of Papilioside I(PPI) on papain-induced osteoarthritis(OA) rats. Methods Thirty healthy male SD rats of the same age were randomly divided into three groups(n=10): model group, PPI low-dose group and PPI high-dose group. Papain was injected into the right knee joint cavity to establish a rat knee joint OA model. After 7 days(d), gastric administration was started. The low and high dose PPI groups were given respectively 2 mg and 5 mg/(kg.d) of saponins I dissolved in DMSO for three consecutive weeks, the model group was given an equal amount of dimethyl sulfoxide(DMSO). After 3 weeks of intragastric intervention, venous blood was collected from each group of rats and the levels of three cytokines(IL-1β, IL-6 and TNF-α) in serum were determined;samples of knee joint cartilage of each group were taken to make frozen sections. And perform HE, Safranin O staining and Mankin score.Results PPI significantly down-regulated the expression of TNF-α, IL-1β and IL-6 in the blood of rats in each treatment group. Compared with the model group, the down-regulation rate of high-dose and low-dose PPI intervention on the three cytokines in rat serum was statistically significant(P<0.05);cartilage surface roughness, fissure formation, and subchondral bone sclerosis, osteophyte hyperplasia, synovial inflammatory changes in OA model group;PPI intervention group cartilage repaired significantly, cartilage surface was relatively smooth, no obvious synovial inflammation and osteophyte hyperplasia. The Mankin score difference between the model group and the PPI low and high dose group was statistically significant(P<0.05), and the difference between the high-dose and low-dose PPI group was statistically significant(P<0.05). Conclusion Papilioside I can improve and repair damaged articular cartilage and reduce inflammation. This study provides experimental evidence for the possibility of Papilioside I as a first-line clinical drug for the treatment of knee osteoarthri

关 键 词：重楼皂苷I(PPI) 骨关节炎 OA大鼠模型 关节软骨 

分 类 号：R684.3[医药卫生—骨科学]