芒果MDS家族基因的鉴定、进化及表达分析  

Gene Identification,Evolution and Expression of MDS Family Genes in Mango(Mangifera indica)

作　　者：盖江涛[1] 王鹏[1] 叶秀旭 罗睿雄[1] 陈业渊[1] Gai Jiangtao;Wang Peng;Ye Xiuxu;Luo Ruixiong;Chen Yeyuan(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海口571101

出　　处：《分子植物育种》2021年第6期1858-1863,共6页Molecular Plant Breeding

基　　金：海南省自然科学基金青年基金项目(319QN310);中国热带农业科学院基本科研业务费专项资金(1630032-019033)共同资助。

摘　　要：2-C-甲基-D-赤藓糖醇-2,4-环焦磷酸合成酶(MDS)是MEP代谢途径中的关键酶。为了鉴定芒果中的MDS家族基因,探索MDS家族基因的进化、表达模式。本研究采用同源搜索的方法,从13个植物基因组中下载了16个MDS家族基因,对其进行了蛋白结构域、系统发育及在芒果中的表达分析。蛋白结构域分析结果显示,芒果MDS家族基因的蛋白序列均包含完整的PF02542.16结构域。系统发育进化树显示,芒果中的MDS家族基因是在双子叶植物形成后进化的,且与柑橘的MDS基因关系最近。表达分析结果显示,在芒果品种‘贵妃’、‘台农’和‘金煌’的成熟果果肉中,芒果MDS家族基因均在‘贵妃’中表达量最高,其次是‘台农’,在‘金煌’中表达量最低,而且差异显著。本研究为进一步研究芒果MDS家族基因在萜类合成途径中的作用提供科学依据。2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase(MDS)is a key enzyme involved in the 2-Cmethyl-D-erythritol-4-phosphate(MEP)pathway.In order to identify the MDS family genes in mango,study the evolution and expression patterns of MDS family genes.In this sutdy,16 MDS family genes were downloaded from 13 plant genomes by homologous search method and carried out the protein domains,phylogeny and the expression analysis of MDS family genes in mango.The results of protein domain analysis showed that PF02542.16 domain in the protein sequences of mango MDS family genes respectively.The construction of phylogenetic tree found that the MDS family genes in mango evolved after the formation of dicotyledon,and are most closely to the MDS gene in Citrus sinensis.The expression analysis showed that the MDS family genes of mango had the highest expression in the flesh of’Guifei’at the ripe stage,the second is’Tainong’,with the lowest expression in’Jinhuang’,and the difference is significant.This research provided a scientific basis for the further analysis of function of mango MDS family genes in terpenoid synthesis pathway.

关 键 词：芒果(Mangifera indica) 2-C-甲基-D-赤藓糖醇-2 4-环焦磷酸合成酶(MDS) MEP 萜类 

分 类 号：S667.7[农业科学—果树学]