不结球白菜形态性状及SSR遗传多样性关联分析  

Association Analysis of Morphological Traits and SSR Genetic Diversity in Non-heading Chinese Cabbage

作　　者：何晓丽 杨丹青 杜志杰 尚春雨 钟凤林[1] He Xiaoli;Yang Danqing;Du Zhijie;Shang Chunyu;Zhong Fenglin(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,350002)

机构地区：[1]福建农林大学园艺学院,福州350002

出　　处：《分子植物育种》2021年第6期1919-1927,共9页Molecular Plant Breeding

基　　金：福州市科技项目(2018-G-37);国家青梗菜良种重大科研联合攻关项目(111821301354052283)共同资助。

摘　　要：分析不结球白菜遗传多样性,筛选与形态性状相关联且具有多态性的标记位点,为不结球白菜的分子辅助育种选择、种质资源的利用和新品种选育提供依据。本研究以类型差异大、有南方特色的54份种质资源为材料,对其6个质量性状和5个数量性状进行3次田间调查,从40对引物中筛选出25对SSR引物进行遗传多样性分析,结合形态性状对54份不结球白菜种质资源进行统计分析。结果表明:6个质量性状间具有丰富的多样性,5个数量性状间有显著的差异性,其中SA变异系数最大,FC与L、W和SA呈显著正相关;25对SSR引物共检测到72个多态性位点,平均每对SSR引物产生2.88个多态性位点,PIC平均值为0.3831,说明不结球白菜SSR具有丰富的遗传多样性;54份不结球白菜的遗传相似系数变化范围为0.492~0.963,平均为0.742,在遗传距离0.6处可将供试材料划分为3个类群;通过关联分析发现5个与数量性状关联的标记位点,各标记的表型变异解释率在0.4657~0.6329,平均为0.5393。本试验获得的5个标记位点可为不结球白菜分子标记育种提供依据。The genetic diversity of non-heading Chinese cabbage was analyzed,and the marker loci associated with morphological traits were screened to provide a basis for molecular marker-assisted selection of morphological traits,utilization of germplasm resources,and breeding of new varieties.Based on 54 types of germplasm resources with large differences and southern characteristics as materials,three field surveys were performed on its6 quality traits and 5 quantitative traits.25 SSR primers were selected from 40 primers for genetic diversity analysis.Based on morphological characteristics,statistical analysis was performed on 54 germplasm resources of non-heading Chinese cabbage.The results showed that there were abundant diversity among 6 quality traits and significant differences among 5 quantitative traits.Among them,the SA coefficient of variation was the largest,and FC was significantly positively correlated with L,W,and SA.A total of 25 SSR primers were detected.72 polymorphic loci,an average of 2.88 polymorphic loci per pair of SSR primers,and an average PIC of 0.3831,indicating that SSR in non-heading Chinese cabbage is rich in genetic diversity;54 non-heading Chinese cabbages are genetically similar.The variation range of the coefficient is 0.492~0.963,with an average of 0.742.The test material can be divided into 3 groups at a genetic distance of 0.6;5 marker sites associated with quantitative traits were found through association analysis,and the phenotypic variation of each marker was explained.The rate is 0.4657~0.6329,with an average of 0.5393.The five marker sites obtained in this experiment can provide basis for molecular marker breeding of non-heading Chinese cabbage.

关 键 词：不结球白菜 形态性状 关联分析 遗传多样性 

分 类 号：S634.3[农业科学—蔬菜学]