多房棘球蚴影响PPARβ、γ表达并调控巨噬细胞极化  

Echinococcus Multilocularis Affects PPARβ、γ Expression and Regulates Macrophage Polarization

作　　者：胡旺 张占红 冯浩杰 崔钰 杜秋沛 于文昊 樊海宁[1,2] HU Wang;ZHANG Zhanhong;FENG Haojie;CUI Yu;DU Qiupei;YU Wenhao;FAN Haining(Department of Hepatobiliary and Pancreatic Surgery,Qinghai University Affiliated Hospital,Xining,Qinghai Province,810001,China;Qinghai Provincial Key Laboratory of Hydatid Disease Research,Xining,Qinghai Province 810001 China)

机构地区：[1]青海大学附属医院肝胆胰外科,青海西宁810001 [2]青海省包虫病研究重点实验室,青海西宁810001

出　　处：《中国高原医学与生物学杂志》2021年第1期1-12,共12页Journal of Chinese High Altitude Medicine & Biology

基　　金：国家重点研发计划“精准医学研究”重点专项(2017YFC0909900);青海省包虫病研究重点实验室项目(2020-ZJ-Y01);青海大学附属医院中青年科研基金项目(2019-QYY-8)。

摘　　要：目的本研究旨在探讨多房棘球蚴影响过氧化物酶体增殖激活受体β和γ(Peroxisome proliferation-activated receptorsβ&γ;PPARβ,γ)表达并调控巨噬细胞极化的状态。方法在体外将RAW264.7巨噬细胞与原头蚴共培养,用qRT-PCR和Western bolt法检测PPARβ、γ在RAW264.7中的表达水平;用qRT-PCR法检测M1和M2相关标记物表达水平来研究PPARβ、γ对巨噬细胞极化的影响。另外,再通过收集25例多房棘球蚴患者手术切除样本,用免疫荧光法检测M1/M2巨噬细胞在正常肝组织和边缘带的面密度值,以及PPARβ、γ在M1/M2巨噬细胞中表达的阳性强度,来进一步验证体外实验结果。结果在巨噬细胞与原头蚴共培养中,巨噬细胞极化标记物的M1型巨噬细胞标记物呈先上升后下降的趋势,而M2型巨噬细胞标记物整体呈上升的趋势。PPARβ、γ的表达分别与M1/M2标记物的变化趋势一致。同时,临床样本分析显示PPARβ、γ的表达分别与M1和M2极化的趋势一致。结论提示PPARβ、γ在多房棘球蚴病中分别参与M1、M2极化调控。Objective The purpose of this study was to investigate the expression of Peroxisome proliferationactivated receptorsβ&γ( PPARβ,γ) to regulate macrophage polarization in alveolar echinococcosis( AE). Methods The expression levels of PPARβ,γ in RAW264.7 macrophages were detected by qRT-PCR and Western Bolt in vitro co-culture.The expression levels of M1 and M2-related markers were detected by qRT-PCR to investigate the effects of PPARβ,γ on the polarization of macrophages.In addition,25 patients with multilocular echinococcsis were collected and the surface density of M1/M2 macrophages in normal liver tissues and liver lesion ranging was detected by immunofluorescence as well as the positive intensity of PPARβ,γ expression in M1/M2 macrophages to further verify the results of the in vitro experiment. Results M1 macrophage polarization markers increased first and then decreased,while M2 markers showed an increasing trend in the co-culture.Simultaneously,the expressions of PPARβ,γ were consistent with the variation of M1 and M2 markers.Meanwhile,the analysis of the clinical samples revealed that the expression of PPARβ,γ were in agreement with the trend of M1 and M2 polarization respectively.Conclusions It is showed that PPARβ,γ may regulate M1 and M2 polarization respectively in AE.

关 键 词：过氧化物酶体增殖激活受体 巨噬细胞 极化 多房棘球蚴病 

分 类 号：R535[医药卫生—内科学]