银屑Ⅰ号含药血清对银屑病样细胞模型的增殖、凋亡相关蛋白及基因的影响  

Effect of Yinxie Ⅰ on Proliferation and Apoptosis-related Proteins and Genes of Psoriasis-like Cell Model

作　　者：陈健勤 邹玲 王莎莉 刘靖[3] 李建华 孙文 CHEN Jianqn;ZOU Ling;WANG Shali;LIU Jing;LI Jianhua;SUN Wen(Integrated Hospital of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510000,China;The First People's Hospital of Jingmen,Jingmen 448000,China;The First Affi-liated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510000,China)

机构地区：[1]南方医科大学中西医结合医院,广东广州510000 [2]荆门市第一人民医院,湖北荆门448000 [3]广州中医药大学第一附属医院,广东广州510000

出　　处：《中国皮肤性病学杂志》2021年第4期444-448,共5页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金(81202699,81573980);湖北省自然科学基金(2018CFB289);湖北省卫生健康委员会2019-2020年度创新团队项目(WJ2019M074);荆门市科学技术研究与开发计划重点项目(2020YFZD022)。

摘　　要：目的探讨银屑Ⅰ号治疗银屑病的药理机制。方法将人永生化角质形成细胞(HaCaT细胞)分为对照组(control组),TNF-α造模组(TNF-α组),中药高剂量组(TNF-α+HD组),中药中剂量组(TNF-α+MD组),中药低剂量组(TNF-α+LD组)。对照组不予造模,其余组别均予TNF-α造模,并予相应血清培养。采用实时荧光定量PCR及蛋白印迹法检测各组Bcl-2、CyclinD1、c-Myc、p21、p53基因及蛋白表达水平。结果对照组的CyclinD1、c-Myc、p21、p53蛋白及mRNA表达水平明显比TNF-α组低,Bcl-2则相反;与TNF-α组相比,TNF-α+LD组、TNF-α+MD组、TNF-α+HD组的CyclinD1、c-Myc、p21、p53蛋白mRNA水平逐渐下降;Bcl-2则相反。结论银屑Ⅰ号可调节一系列增殖、凋亡相关蛋白及基因,进而调节角质形成细胞增殖凋亡代谢网络,从而发挥抑制细胞增殖、促进细胞凋亡的作用。Objective To explore the pharmacological mechanism of YinxieⅠin the treatment of psoriasis.Methods Human immortalized keratinocytes(HaCaT cells)were divided into control group(control group),a TNF-αmodel group(TNF-αgroup),high dose group(TNF-α+HD group),medium dose group(TNF-α+MD group)and low dose group(TNF-α+LD group).The control group was given no modeling,and the other groups were given TNF-modeling and corresponding serum culture.The control group was not modeled,and the other groups were modeled with TNF-αand cultured with corresponding serum.Real-time fluorescent quantitative PCR and Western blotting were used to detect the expression levels of Bcl-2,CyclinD1,c-Myc,p21,and p53 genes and proteins in each group.Results The expression levels of CyclinD1,c-Myc,p21,p53 protein and mRNA in the control group were significantly lower than those in the TNF-αgroup,while Bcl-2 was the opposite.Compared with the TNF-αgroup,the CyclinD1,c-Myc,p21,and p53 protein mRNA levels in the TNF-α+LD group,TNF-α+MD group,and TNF-α+HD group gradually decreased;Bcl-2 was the opposite.Conclusion YinxieⅠcan regulate a series of proliferation and apoptosis-related proteins and genes,and then regulate keratinocytes proliferation and apoptosis metabolic network,so as to play the role of inhibiting cell proliferation and promoting cell apoptosis.

关 键 词：银屑病 人永生化角质形成细胞 银屑Ⅰ号 增殖 凋亡 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]