肉桂配伍“黄柏-知母”药对对肾盂肾炎大鼠炎症因子的影响  

Effects of Cinnamomum Cassia Presl Combined with“Phellodendri Chinensis-Anemarrhenae Rhizoma”on Inflammatory Factors in Rats with Pyelonephritis

作　　者：王迅 刘顺 邹恺平 刘月恒 朱广平 WANG Xun;LIU Shun;ZOU Kaiping;LIU Yueheng;ZHU Guangping(Dept.of Preparation,Affiliated Hospital of Nanjing University of Chinese Medicine,Jiangsu Nanjing,210029)

机构地区：[1]南京中医药大学附属医院制剂部,江苏南京210029

出　　处：《中国医院用药评价与分析》2021年第3期257-261,268,共6页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：国家自然科学基金项目(No.81603310)。

摘　　要：目的:探讨肉桂配伍"黄柏-知母"药对对肾盂肾炎大鼠肾脏及血清样本中炎症因子的影响,初步探讨肉桂反佐配伍治疗肾盂肾炎的分子机制。方法:建立肾盂肾炎大鼠模型,设立对照组、模型组、阳性药组(采用盐酸左氧氟沙星)、滋肾通关方组(采用肉桂配伍"黄柏-知母"药对)及药对组(采用单纯"黄柏-知母"药对)进行比较;以酶联免疫吸附试剂盒检测炎症因子[白细胞介素(IL)1α、IL-1β、肿瘤坏死因子α(TNF-α)、IL-6、单核细胞趋化蛋白1(MCP-1)、CXCL2]在各组大鼠血清中的含量,进行药效学考察。结果:(1)阳性药组、滋肾通关方组及药对组大鼠机体状况均有改善。通过比较病理切片,滋肾通关方组与阳性药组大鼠的改善情况明显优于"黄柏-知母"药对组。(2)模型组大鼠的IL-1α、IL-1β、TNF-α、IL-6、MCP-1及CXCL2含量均较对照组更高,而且模型组大鼠血清IL-α、CXCL2含量与对照组比较,差异均有统计学意义(P<0.05)。(3)阳性药组、滋肾通关方组及药对组大鼠体内炎症因子含量相对模型组均有降低;其中,滋肾通关方组大鼠血清IL-1α含量、阳性药组大鼠血清TNF-α含量与模型组的差异均有统计学意义(P<0.05)。结论:"黄柏-知母"药对反佐配伍少量肉桂后,能够促进黄柏、知母中活性成分下调IL-1α、IL-1β、TNF-α、IL-6、MCP-1及CXCL2等炎症因子的表达,影响下游炎症因子的免疫应答,进而起到抗炎以及保护肾脏等泌尿系统器官的作用。OBJECTIVE:To probe into the effects of Cinnamomum cassia Presl combined with"Phellodendri Chinensis-Anemarrhenae Rhizoma"on inflammatory factors in kidney and serum samples of rats with pyelonephritis,and to explore the molecular mechanism of Cinnamomum cassia Presl in the treatment of pyelonephritis.METHODS:Rat model of pyelonephritis was established,the control group,model group,positive drug group(levofloxacin hydrochloride),Zishen Tongguan prescription group(Cinnamomum cassia Presl combined with"Phellodendri Chinensis-Anemarrhenae Rhizoma")and drug combination group("Phellodendri Chinensis-Anemarrhenae Rhizoma")were compared.Serum concentrations of inflammatory factors[interleukin(IL)1α,IL-1β,tumor necrosis factorα(TNF-α),IL-6,monocyte chemotaxis protein 1(MCP-1),CXCL2]in each group were determined by ELISA for pharmacodynamic study.RESULTS:(1)The disease condition of rats in the positive drug group,Zishen Tongguan prescription group and drug combination group was improved.Through the comparison of pathological sections,the improvement of rats in the Zishen Tongguan prescription group and the positive drug group was significantly better than that of the"Phellodendri Chinensis-Anemarrhenae Rhizoma"drug combination group.(2)The levels of IL-1α,IL-1β,TNF-α,IL-6,MCP-1 and CXCL2 in the model group were higher than those in the control group,and there were statistically significant differences in the levels of IL-1αand CXCL2 in serum of the model group and the control group(P<0.05).(3)Compared with model group,the levels of inflammatory factors in the positive drug group,Zishen Tongfang prescription group and drug combination group decreased;compared with the model group,there were significant differences in levels of IL-1αin the Zisheng Tongfang prescription group and levels of TNF-αin the positive drug group(P<0.05).CONCLUSIONS:"Phellodendri Chinensis-Anemarrhenae Rhizoma"combined with Cinnamomum cassia Presl can promote the active components of Phellodendri Chinensis and Anemarrhenae Rhizoma to down-re

关 键 词：滋肾通关方 反佐 肾盂肾炎 炎症因子 

分 类 号：R932[医药卫生—生药学]