颐脑解郁方对卒中后焦虑大鼠炎性因子及p38-MAPK信号蛋白表达的影响  

Effects of Yinao Jieyu Formula on the Expression of Inflammatory Factors and p38-MAPK Signal Protein in Post-stroke Anxiety Rats

作　　者：寇娜[1,2] 郝钰 赵瑞珍[2] 赵子珺 王丹 唐启盛 KOU Na;HAO Yu;ZHAO Ruizhen;ZHAO Zijun;WANG Dan;TANG Qisheng(Beijing University of Chinese Medicine,Beijing 100029,China;Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China)

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学第三附属医院,北京100029 [3]北京中医药大学东直门医院

出　　处：《中西医结合心脑血管病杂志》2021年第6期945-948,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家自然科学基金项目(No.81473658);国家中医药管理局“岐黄学者”项目。

摘　　要：目的探讨炎性因子及p38丝裂原活化蛋白激酶(p38-MAPK)信号蛋白与卒中后焦虑(PSA)的关系及颐脑解郁方的干预作用。方法采用不确定性空瓶饮水刺激复合脑缺血建立卒中后焦虑大鼠模型,随机分为模型组、治疗组,另设正常组、卒中组,每组15只。治疗组大鼠灌胃中药颐脑解郁方,正常组、卒中组、模型组大鼠常规饲养,灌胃生理盐水,连续灌胃4周。大鼠末次灌胃行旷场实验及高架十字迷宫相关检测后每组随机选择12只大鼠进行腹主动脉取血,采用放射免疫法检测血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1α(IL-1α)、白细胞介素-6(IL-6)的含量;每组随机选择6只大鼠采用蛋白免疫印迹(Western Blot)法检测大鼠右侧(病侧)皮质及海马p38-MAPK蛋白的表达。结果旷场试验及高架十字迷宫结果显示,与正常组比较,卒中组水平及垂直运动次数减少(P<0.01),模型组、治疗组的水平及垂直运动次数、开放臂次数及时间均明显减少(P<0.05或P<0.01);与卒中组比较,模型组水平及垂直运动次数、开放臂次数及时间均减少(P<0.01);与模型组比较,治疗组水平及垂直运动次数、开放臂次数及时间均明显升高(P<0.05或P<0.01)。放射免疫法检测结果显示,与正常组比较,卒中组、模型组、治疗组大鼠血清TNF-α、IL-1α、IL-6含量升高(P<0.01);与卒中组比较,模型组大鼠血清TNF-α、IL-1α、IL-6含量明显升高(P<0.05或P<0.01);与模型组比较,治疗组大鼠血清TNF-α、IL-1α、IL-6含量降低(P<0.01)。Western Blot检测结果显示,与正常组比较,模型组大鼠海马及皮质p38-MAPK表达升高(P<0.01);与卒中组比较,模型组大鼠海马p38-MAPK表达明显升高(P<0.05);与模型组比较,治疗组大鼠海马及皮质p38-MAPK表达下降(P<0.01)。结论颐脑解郁方可改善脑卒中后引起的焦虑障碍,其机制可能与抑制下丘脑和皮质p38-MAPK磷酸化水平,减轻炎性因子应答相关。Objective To explore the relationship between inflammatory factors,p38 mitogen activated protein kinase(p38-MAPK)signal protein and post-stroke anxiety(PSA),and to further clarify the therapeutic effect of Yinao Jieyu formula on PSA.Methods The rat model of PSA was established by the method of uncertain empty bottle water stimulation.The rats were randomly divided into model group,treatment group,normal group,and stroke group,with 15 rats in each group.Rats in treatment group were given Yinao Jieyu formula by gavage.Rats in normal group,stroke group,and model group were fed with 0.9%normal saline by gavage for 4 weeks.After the last open field test and elevated cross maze test,12 rats in each group were randomly selected to take blood from abdominal aorta.The serum tumor necrosis factor-α(TNF-α),interleukin-1α(IL-1α),and interleukin-6(IL-6)were detected by radioimmunoassay.Western blot was used to detect the expression of p38-MAPK protein in the right cortex and hippocampus.Results The results of field test and elevated cross maze showed that compared with normal group,the number of vertical movements in stroke group decreased(P<0.01),the number of horizontal and vertical movements,the number of open arms,and the times and time of opening arms in model group and treatment group significantly reduced(P<0.05 or P<0.01);Compared with stroke group,the times of horizontal and vertical movement,the number and time of open arm in model group significantly reduced(P<0.01).Compared with model group,the times of horizontal and vertical movement,the times and time of opening arms in treatment group significantly increased(P<0.05 or P<0.01).The results of radioimmunoassay showed that compared with normal group,the levels of serum TNF-α,IL-1α,and IL-6 in stroke group,model group,and treatment group significantly increased(P<0.01);Compared with stroke group,the serum TNF-αand IL-1αcontents of model group significantly increased(P<0.05 or P<0.01);Compared with model group,the serum TNF-αand IL-6 contents of treatment g

关 键 词：脑卒中 焦虑 颐脑解郁方 炎性因子 P38丝裂原活化蛋白激酶 实验研究 

分 类 号：R285.5[医药卫生—中药学]