基于整合药理学探究益气明目颗粒治疗气虚血瘀型青光眼分子作用机制  

Molecular Mechanism of Yiqi Mingmu Granules in Treatment of Glaucoma with Qi Deficiency and Blood Stasis Based on Integrated Pharmacological Technology

作　　者：陈文黎 张丽霞[1] 倘孟莹 陈爽 CHEN Wenli;ZHANG Lixia;TANG Mengying;CHEN Shuang(Eye Hospital,China Academy of Chinese Medical Sciences,Beijing 100040,China)

机构地区：[1]中国中医科学院眼科医院,北京100040

出　　处：《中国中医药信息杂志》2021年第4期26-31,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(82074500);首都卫生发展科研专项(首发2018-1-4181)。

摘　　要：目的采用整合药理学技术探究益气明目颗粒治疗气虚血瘀型青光眼的分子作用机制。方法依托中医药整合药理学研究平台(TCMIP)V2.0收集益气明目颗粒7味中药的化学成分及其对应靶点,以及气虚血瘀型青光眼的靶点信息,构建药物与疾病的核心靶点网络。借助DAVID6.8进行GO功能分析和KEGG通路富集分析,构建“中药-成分-靶点-通路”多维网络,对主要成分进行ADMET水平分析和类药性定量评估(QED)。结果共获得益气明目颗粒282个化学成分,对应靶点815个,气虚血瘀型青光眼相关靶点571个。经药物和疾病靶点蛋白相互作用网络分析,选取前150个节点作为核心靶点。核心靶点GO分析结果显示其主要作用于RNA聚合酶Ⅱ启动子转录的正调控、转录和DNA模板、ATP结合、DNA结合等,KEGG通路分析显示其主要富集于Rap1信号通路、HIF-1信号通路、PI3K-Akt信号通路等。多维网络及QED显示,主要作用靶点有TNF-α、IL-6、TLR4、NFKB1、AKT等,参与的主要成分有Daidzein、Azelaic Acid、Sebacic Acid等。结论益气明目颗粒可能通过改善视盘缺氧、降低炎症介质水平、调节线粒体能量代谢等治疗气虚血瘀型青光眼。Objective To study the molecular mechanism of Yiqi Mingmu Granules in the treatment of glaucoma with qi deficiency and blood stasis through integrated pharmacological technology.Methods Compounds of 7 types of Chinese materia medica and the according targets in Yiqi Mingmu Granules and the target information of glaucoma with qi deficiency and blood stasis were collected based on TCMIP V2.0 to construct the core target network of drugs and diseases.With the help of DAVID6.8 for GO function analysis and KEGG pathway enrichment analysis,a multi-dimensional network diagram of“Chinese materia medica-components-target-pathways”was constructed.The levels of ADMET and QED of the main components in the network were analyzed.Results A total of 282 chemical components and 815 targets in Yiqi Mingmu Granules were obtained.A total of 571 targets of glaucoma with qi deficiency and blood stasis were obtained.The interaction network analysis of drug and disease target protein selected the first 150 nodes as core targets.The core target GO analysis results showed that it mainly acted on the positive regulation of RNA polymeraseⅡpromoter transcription,transcription and DNA template,ATP binding,DNA binding,etc.KEGG pathway analysis showed that it was mainly enriched in Rap1 signaling pathway,HIF-1 signaling pathway,PI3K-Akt signaling pathway,etc.Multi-dimensional network diagram and QED showed that the main targets were TNF-α,IL-6,TLR4,NFKB1,AKT,etc.The main components involved were Daidzein,Azelaic Acid,Sebacic Acid,etc.Conclusion Yiqi Mingmu Granules may treat glaucoma with qi deficiency and blood stasis by improving optic disc hypoxia,reducing the level of inflammatory mediators,and regulating mitochondrial energy metabolism.

关 键 词：青光眼 气虚血瘀 益气明目颗粒 整合药理学 分子机制 多维网络 

分 类 号：R276.752[医药卫生—中医五官科学] R285[医药卫生—中医学]