miR-637靶向AKT3对甲状腺乳头状癌TPC-1细胞侵袭和转移能力的影响  

Effects of miR-637 on invasion and metastasis capacity of thyroid cancer TPC-1 cells by regulating AKT3 expression

作　　者：袁青领[1] 樊玉霞[1] 刘洋[1] 王晓明[1] 刘征[1] 贾勐[1] 耿祖仕[1] 郑建[1] 卢秀波[1] YUAN Qingling;FAN Yuxia;LIU Yang;WANG Xiaoming;LIU Zheng;JIA Meng;GENG Zushi;ZHENG Jian;LU Xiubo(Department of Thyroid Surgery, the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052)

机构地区：[1]郑州大学第一附属医院甲状腺外科,郑州450052

出　　处：《郑州大学学报（医学版）》2021年第2期149-153,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省医学科技攻关计划(联合共建)项目(LHGJ20190150)。

摘　　要：目的:探讨甲状腺乳头状癌TPC-1细胞中miR-637和AKT3基因的靶向关系,及miR-637对TPC-1细胞侵袭转移能力的影响。方法:利用生物信息学软件预测miR-637与AKT3之间的靶向关系,并采用双荧光素酶报告实验进行验证。TPC-1细胞分别转染miR-637 mimic(miR-637组)和miR-637阴性对照(阴性对照组),以不转染细胞作空白对照。Western blot法检测3组细胞中AKT3蛋白的相对表达量。利用划痕实验及Transwell侵袭实验检测空白对照和miR-637组细胞的迁移及侵袭能力。结果:双荧光素酶报告实验证实miR-637能够结合AKT3 mRNA 3’UTR(P<0.001)。miR-637组细胞中AKT3蛋白相对表达量显著低于空白对照组和阴性对照组(P<0.001);miR-637组细胞迁移率和穿膜细胞数均低于空白对照组(P<0.001)。结论:miR-637通过靶向负调控AKT3基因的表达,抑制TPC-1细胞的侵袭和迁移能力。Aim:To investigate the relation between miR-637 and AKT3,and its influence on invasion and metastasis of papillary thyroid carcinoma cell TPC-1.Methods:The target matching relationship between miR-637 and AKT3 was predicted by bio-informatic software,and was identified by double luciferase reporter system.TPC-1 cells were transfected with miR-637 mimic(miR-637 group)and miR-637 negative control(negative control group),respectively.The cells without transfection were the blank control.The relative expression of AKT3 protein in the 3 groups was detected by Western blot.The migration and invasive capacity of the cells in blank control group and miR-637 group were measured by scratch test and Transwell invasive test,respectively.Results:Double luciferase reporter system identified that miR-637 could bind AKT3 mRNA 3’UTR(P<0.001).The AKT3 protein expression in miR-637 group was lower than those in negative control group and blank control group(P<0.001).The migration rate and invasive capacity of the cells in miR-637 group were lower than those of blank control group(P<0.001).Conclusion:The miR-637 may negatively regulate AKT3 gene expression and inhibit the invasion and metastasis of TPC-1 cells.

关 键 词：微小RNA-637 AKT3 侵袭转移 甲状腺乳头状癌 

分 类 号：R736.1[医药卫生—肿瘤]