儿童幽门螺杆菌感染后的胃内菌群结构分析  

Analysis of the Structure of the Gastric Microbiota in Children after Helicobacter Pylori Infection

作　　者：汤志鸿 蒋丰智[1] 黎小秀[1] 段高羊 廖琼 TANG Zhi-hong;JIANG Feng-zhi;LI Xiao-xiu(Department of Pediatrics,Dongguan Materoai and Child Heclth Care Hospital,Dongguan,Guangdong,523000,China)

机构地区：[1]东莞市妇幼保健院儿科,广东东莞523000

出　　处：《黑龙江医学》2021年第7期689-691,共3页Heilongjiang Medical Journal

基　　金：东莞市社会科技发展一般项目(2018507150071527)。

摘　　要：目的:比较儿童幽门螺杆菌(HP)感染后胃微生物菌群结构的变化及其在发病机制中的作用。方法:收集东莞市妇幼保健院门诊及住院部有消化道临床症状而接受胃镜检查的患儿40人(HP阳性组26例,HP阴性组14例),钳取胃黏膜标本用于快速尿素酶试验、组织病理学检查、DNA提取,分析HP阳性组和HP阴性组两组之间的社会人口资料学、体重指数、尿素呼气试验、病理组织结果等临床特征,通过16S rDNA高通量测序技术比较HP阳性组和HP阴性组患儿胃内微生物菌群结构。结果:HP阳性组体重过低(BMI<18.5 kg/m^(2))的患儿比例为69.2%,非体重过低(BMI>18.5 kg/m^(2))的患儿比例为30.3%(P=0.04),幽门螺杆菌感染组患儿体重过低,差异有统计学意义。病理组织结果显示,HP阳性组活动性浅表性胃炎、出血性胃炎、胃溃疡的比例高于HP阴性组,差异有统计学意义(P=0.01)。HP阴性组的物种丰度和物种多样性高于HP阳性组,差异有统计学意义(P=0.001)。HP阳性组Prevotella普氏菌属(7%VS3%)、Fusobacterium梭杆菌属(6%VS3%)比例较HP阴性组高。结论:幽门螺杆菌感染影响儿童体重、胃内病理组织、胃黏膜菌群结构,HP感染后胃黏膜菌群的多样性降低,幽门螺杆菌可能与胃内菌群共同促进胃部疾病的发生、发展。Objective:To compare the changes in the structure of gastric microbiota and its role in the pathogenesis of Helicobacter pylori(HP)infection in children.Methods:40 children who underwent gastric endoscopy with dyspeptic symptoms(26 H.pylori positive and 14 negative)were selected.Gastric biopsies were obtained for rapid urease test,histopathological examination and DNA extraction.Social demographics,BMI,urea breath test,pathological tissue results and other clinical features between HP positive group and HP negative group were analyzed.The structure of the microbial flora in children’s stomach of two groups was compared by 16 s rDNA high-throughput sequencing.Results:In the HP positive group,the proportion of children with underweight(BMI<18.5 kg/m^(2))was 69.2%,and the proportion without underweight(BMI>18.5 kg/m^(2))was 30.3%.The children in the helicobracter infection group were underweight,with statistically significant difference.The histopathological results showed that the proportion of active superficial gastritis,hemorrhagic gastitis and gastric ulcer in the HP positive group was higher than that in the HP nigative group,with statistically significant difference(P=0.01).Bacterial richness and diversity of H.pylori-positive specimens were lower than those in H.pylori-negative.Active superficial gastritis,hemorrhagic gastritis,gastric ulcer in H.pylori-positive children were higher than those in H.pylori-negative children(P=0.01).The relative abundance of Prevotella and Fusobacterium in H.pylori-positive children were higher than H.pylori-negative children.Conclusion:H.Pylori can significantly affect the structure and composition of gastric microbiota.H.Pylori and surrounding gastric microbiota may jointly promote the development of gastric diseases.

关 键 词：儿童幽门螺杆菌感染 胃微生物菌群 16S rDNA高通量测序技术 

分 类 号：R446.1[医药卫生—诊断学]