先天性心脏病突变体小鼠模型的建立  

Generation of Congenital Heart Disease Mutant Mouse Model

作　　者：尹梓豪 孙一兵[1] 向寿莲 张健[1] 丁小凤[1] 李立民 YIN Zi-hao;SUN Yi-bing;XIANG Shou-lian;ZHANG Jian;DING Xiao-feng;LI Li-min(College of Life Sciences,Hunan Normal University,Changsha 410081,China;College of Engineering and Design,Hunan Normal University,Changsha 410081,China)

机构地区：[1]湖南师范大学生命科学学院,中国长沙410081 [2]湖南师范大学工程设计学院,中国长沙410081

出　　处：《湖南师范大学自然科学学报》2021年第2期48-53,共6页Journal of Natural Science of Hunan Normal University

基　　金：国家自然科学基金项目(81872256);湖南省教育厅重点项目(19A310)。

摘　　要：为研究AP-2α在发育过程中的特定调控作用,利用AP-2α-LacZ转基因小鼠FX18研究AP-2α与先天性心脏病的相关性。在FX18小鼠繁殖过程中,发现该小鼠稳定遗传的突变体,鉴定其基因型,测量体质量与心脏质量,HE染色和心电图观察其表型和检测心脏功能。研究结果表明:突变体小鼠在出生后4周左右即死亡,且在胚胎期就表现出明显的发育迟缓,出生后的体质量和心脏质量均仅为正常FX18小鼠的40%~60%,同时,HE染色和心电图检测结果显示,小鼠表现出较明显的左心室肥大和室性早搏,符合先天性心脏病中动脉导管未闭的特征。因此,成功发现和建立的该突变体小鼠可作为一个较好的研究先天性心脏病小鼠模型。To study the specific regulatory role of AP-2αduring development,AP-2α-LacZ transgenic mice FX18 were obtained to investigate the correlation between AP-2αand congenital heart disease.During the reproduction of FX18 mice,stable genetic mutants were found.FX18 mice were genotyped.Mouse body weight and heart weight were measured.Their phenotypes were observed by HE staining,and electrocardiogram was performed to examine the heart function.Our results revealed that mutant mice died about 4 weeks after birth and showed significant developmental retardation at the embryonic stage.Both postnatal body weight and heart weight of mutant mice were only 40%—60%of normal FX18 mice.At the same time,the results of HE staining and electrocardiogram indicated that the mice had obvious left ventricular hypertrophy and ventricular premature beat,which were characteristics of patent ductus arteriosus in congenital heart disease.Therefore,in this work,we successfully identified and established this mutant mouse,which could serve as a better model for studying congenital heart disease in mice.

关 键 词：转基因小鼠FX18 AP-2Α 突变体小鼠 先天性心脏病 

分 类 号：R541.1[医药卫生—心血管疾病] R-332[医药卫生—内科学]