针刺对戊四氮致痫大鼠抗癫痫机制的研究  

Anti-Epileptic Mechanism of Acupuncture in Treating Epilepsy Rats Induced by PTZ

作　　者：何青 张齐娟 甘学文 曹庭欣 HE Qing;ZHANG Qijuan;GAN Xuewen;CAO Tingxin(Hubei 672 Orthopaedic Integrated Hospital of Traditional Chinese and Western Medicine, Wuhan 430000, China)

机构地区：[1]湖北六七二中西医结合骨科医院,湖北武汉430000

出　　处：《针灸临床杂志》2021年第1期75-79,共5页Journal of Clinical Acupuncture and Moxibustion

基　　金：武汉市卫生厅科研资助项目,编号:WZ16D18。

摘　　要：目的:探讨针刺对戊四氮(PTZ)致痫大鼠抗癫痫的作用机制。方法:将实验大鼠分为NS组、PTZ组和针刺组,造模后观察各组大鼠癫痫发作的行为学表现,采用免疫组化法检测大脑皮质及海马caspase-3在36 h和72 h时间点免疫反应性,采用半定量RT-PCR法检测海马bcl-2、bax蛋白表达。结果:针刺组大鼠癫痫发作较PTZ组减轻;在36 h时间点,PTZ组大鼠大脑皮质及海马caspase-3免疫反应性、海马bax mRNA的表达高于NS组(P<0.05),针刺治疗可拮抗其高表达(P<0.05),Bcl-2与Bax蛋白的表达水平呈负相关,在72 h时间点各组之间差异更为明显。结论:PTZ致痫诱导大鼠海马神经元凋亡,针刺PTZ致痫大鼠百会、大椎穴可减轻癫痫发作程度,可能通过抑制凋亡途径进而对神经元发挥神经保护产生抗癫痫效应。Objective:To explore the anti-epileptic mechanism of acupuncture in treating epilepsy rats induced by pentylenetetrazol(PTZ).Methods:The experimental rats were randomly divided into the blank group,the model group and the acupuncture group.The behavior of the rats were observed during epilepsy seizure.The immunoreactivity of caspase-3 in cerebral cortex and hippocampus was detected by immunohistochemistry at 36 h and 72 h after model establishment in each group,and the protein expressions of Bax and Bcl-2 were detected by semi-quantitative RT-PCR.Results:The degree of seizures in the acupuncture group was significantly weaker than that in the model group.At the time point of 36 h,the immunoreactivity and the level of Bax mRNA in the cerebral cortex and hippocampus were significantly higher in the model group than those in the control group(P<0.05).Acupuncture could antagonize the high immunoreactivity and the high expression(P<0.05).The expression level of Bcl-2 was negatively correlated with the expression of Bax.The differences were more obvious at the time point of 72 h.Conclusion:Epilepsy induced by PTZ can induce apoptosis of hippocampal neurons in rats.Needling Baihui(DU20)and Dazhui(DU7)can alleviate the degree of epilepsy seizures,and exert an anti-epileptic effect by protecting neurons and inhibiting apoptosis.

关 键 词：癫痫 针刺 凋亡 戊四氮 大鼠 海马 

分 类 号：R246.6[医药卫生—针灸推拿学]