OPN基因多态性与潮汕人群女性SLE发病风险的相关性  

Association between osteopontin genetic polymorphism and the risk of systemic lupus erythematosus in Chaoshan women

作　　者：陈新瑶 丁燕佳 梁雪雁 黄慧莹 韦华贵 王俊利 林敏[3] CHEN Xinyao;DING Yanjia;LIANG Xueyan;HUANG Huiying;WEI Huagui;WANG Junli;LIN Min(Department of Medical Laboratory,Chaozhou People's Hospital Affiliated to Shantou University Medical College,Chaozhou,Guangdong,China,521011;School of Clinical Medicine,Youjiang Medical Univer-sity for Nationalities,Baise,Guangxi,China,533000;School of Food Engineering and Biotechnology,Hanshan Normal University,Chaozhou,Guangdong,China,521011)

机构地区：[1]汕头大学医学院附属潮州市人民医院中心实验室,广东潮州521011 [2]右江民族医学院检验学院,广西百色533000 [3]韩山师范学院食品工程与生命科学院,广东潮州521011

出　　处：《分子诊断与治疗杂志》2021年第3期360-363,367,共5页Journal of Molecular Diagnostics and Therapy

基　　金：广东省医学科学技术研究基金项目(B2019232);广西省自然基金重点项目(AB18221029,2019JJD140052);广东省教育厅重点项目(2019-GDXK-0031)。

摘　　要：目的探讨骨桥蛋白(OPN)基因三个单核苷酸多态性(SNP)位点rs1126772、rs11728697和rs4754与潮汕人群女性系统性红斑狼疮(SLE)发病风险的相关性。方法选取2018年1月至2019年12月在汕头大学医学院附属潮州市人民医院以及汕头大学医学院第一附属医院就诊的59例SLE女性患者作为研究组,选取同期94例健康人作为对照组,采用PCR-Sanger测序与高分辨率熔解曲线(HRM)分析法确认rs1126772、rs11728697和rs4754的基因型,Logistic回归分析SNP与SLE的相关性。结果研究组和对照组中rs1126772、rs11728697和rs4754位点的基因型分布比较差异无统计学意义(P>0.05),与SLE的临床症状无关(P>0.05)。结论潮汕人群女性OPN基因的rs1126772、rs11728697和rs4754位点与SLE发病风险无关。Objective To explore the relationship between the three single nucleotide polymorphism(SNP)sites rs1126772,rs11728697 and rs4754 of the osteopontin(OPN)gene and the risk of systemic lupus erythematosus(SLE)in Chaoshan women. Method A total of 59 female SLE patients treated at Chaozhou People.s Hospital Affiliated to Shantou University Medical College and the First Affiliated Hospital of Shantou University Medical College from January 2018 to December 2019 were recruited as the research group. 94 healthy people during the same period were selected as the control group. PCR-Sanger sequencing and high-resolution melting curve(HRM) analysis were used to confirm the genotypes of rs1126772,rs11728697 and rs4754,and the correlation between SNP and SLE was analyzed by Logistic regression.Results There was no significant difference in the genotype distribution of rs1126772,rs11728697 and rs4754 between the research group and the control group(P>0.05)and it was not associated with the clinical symptoms of SLE(P>0.05). Conclusion The rs1126772,rs11728697 and rs4754 loci of OPN gene in Chaoshan women are not associated with the risk of SLE.

关 键 词：骨桥蛋白 系统性红斑狼疮 单核苷酸多态性 

分 类 号：R593.241[医药卫生—内科学]