HBV病毒慢性感染者的ALDH2基因多态性研究  

Study on ALDH2 Gene Polymorphism in Patients with Chronic HBV Virus Infection

作　　者：赵婧[1] 岳鹏 邢国征 尹显艳 于洪远[1] ZHAO Jing;YUE Peng;XING Guozheng;YIN Xianyan;YU Hongyuan(Department of Clinical Laboratory,Beijing Aerospace General Hospital,Beijing,China,100076;The Yang Fang Clinic,The Northern Medical District of the PLA General Hospital,Beijing,China,102205)

机构地区：[1]北京航天总医院检验科,北京市100076 [2]解放军总医院京北医疗区阳坊门诊部,北京市102205

出　　处：《分子诊断与治疗杂志》2021年第3期368-371,共4页Journal of Molecular Diagnostics and Therapy

基　　金：首都临床特色应用研究与成果推广(Z171100001017131);北京航天总医院创新基金科研立项(2020-610)。

摘　　要：目的探讨慢性乙型肝炎患者中ALDH2基因504位点多态性分布及其与HBV感染的相关性。方法选取2014年1月至2020年1月之间于本院就诊的125例慢性乙肝患者作为研究组,根据临床分型的不同,分为CHB I级72例,CHB II级47例,CHB III级6例,同期健康体检者60名为对照组。收集标本,采用基因芯片法检测ALDH2基因多态性,SPSS 23.0软件分析各组数据的相关性。结果CHB患者不同临床分型与血清HBV-DNA病毒载量比较,差异有统计学意义(P<0.05);血清HBV-DNA病毒载量与ALDH2基因分型比较,差异无统计学意义(P=0.068);GA+AA型与GG型基因在研究组与对照组中分布比较,差异有统计学意义(P=0.042),在CHB临床分型中差异无统计学意义(P>0.05);经Logistic回归分析GA+AA型与GG型基因HBV感染风险的差异有统计学意义(P<0.05);对吸烟和饮酒状况进行校正后,GA+AA型的乙肝病毒感染风险有所增加(OR=1.939,95%CI:1.028~3.658)。结论乙肝病毒的感染与ALDH2基因504位点的单核苷酸多态性有关,突变型为其感染危险因素。Objective To explore the distribution of ALDH2 gene 504-locus polymorphism in patients with chronic hepatitis B and its correlation with HBV infection. Methods A total of 125 patients with CHB who were treated in our hospital from Jan 2014 to Jan 2020 were enrolled in the study and divided into the CHB I group with 72 mild cases,the CHB II group with 47 moderate cases and the CHB III group with 6 severe cases according to the clinical classification,meanwhile,60 healthy people who received physical examination were set as the control group. The genotype distribution was detected by gene chip,and the data were analyzed by SPSS 23.0. Results There was statistically significant difference between the serum HBVDNA viral load and the different clinical types of CHB patients(P<0.05),but no significant difference between the serum HBV-DNA viral load and ALDH2 genotyping(P=0.068). The GA+AA type and GG type genes were compared in the study group and the control group,the difference was statistically significant(P=0.042),and there was no statistically significant difference in the clinical classification of CHB(P>0.05). Logistic regression analysis showed that the difference of HBV infection risk between GA+AA type and GG type gene was statistically significant(P<0.05). After adjusting for smoking and drinking status,the risk of GA+AA type of hepatitis B virus infection increased(OR=1.939,95%CI:1.028~3.658). Conclusion The infection of hepatitis B virus is related to the single nucleotide polymorphism at 504 of ALDH2 gene,and the mutation is its risk factor for infection.

关 键 词：乙型肝炎 乙醛脱氢酶基因 多态性 分子诊断 

分 类 号：R512.62[医药卫生—内科学]