一例HIV窗口期样本三种试管核酸检测结果分析  

Analysis of NAT results of one HIV window period sample by three processing modes

作　　者：帅敏[1] 薛薇[1] 黄超 邹晨 田智[1] SHUAI Min;XUE Wei;HUANG Chao;ZOU Chen;TIAN Zhi(Department of Clincal Laboratory,Leshan blood center,Leshan,Sichuan,China,614000)

机构地区：[1]乐山市中心血站检验科,四川乐山614000

出　　处：《分子诊断与治疗杂志》2021年第3期430-432,436,共4页Journal of Molecular Diagnostics and Therapy

摘　　要：目的通过对HIV窗口期样本三种类型真空采血管核酸检测结果的分析,探讨三种类型真空采血管样本对HIV窗口期样本核酸检测结果的影响。方法分析一例2018年7月30日在马边县采集的无偿献血者三种类型真空采血管HIV窗口期样本的核酸检测结果。第一种是带分离胶抗凝真空采血管5℃低温离心20 min;第二种是带分离胶促凝真空采血管25℃常温离心10 min;第三种是抗凝真空采血管(无分离胶)25℃常温离心5 min。对3份样本进行核酸检测。后续完成该样本的病毒载量检测,由市CDC完成献血者追踪和重新采样进行HIV确认。结果核酸检测结果是:第一种模式Ct值为32.4,第二种模式Ct值为32.69,第三种模式Ct值为32.76;病毒载量分析结果为3.28×103IU/mL;三个月后CDC重新采样确认为HIV阳性。结论试管类型对HIV窗口期样本核酸检测结果影响较小,足量无溶血无脂血的三种类型试管的样本都可以用于核酸检测。Objective Objective to analyze the nucleic acid detection results of three types of vacuum blood collection in HIV window period samples,to investigate the effect of different treatment conditions of three types of vacuum blood collection samples on nucleic acid detection results of HIV window period samples.Methods To analysis of NAT results of HIV window samples processed in three different modes,three samples collected from a unpaid blood donor in Mabian County on July 30,2018.The first mode is 5℃cryogenic centrifugation 20-minute separation adhesive anticogulant tube.The second mode is 25℃normal temperature centrifugation 10-minute separation adhesive procoagulant tube.The third mode is 25℃normal temperature centrifugation 5-minute anticoagulant tube(no separation adhesive).Three HIV window period samples to NAT simultaneous.After completing the Riral load test of the sample,completion of HIV confirmation experiment by CDC.Results The results of NAT 77 hours after sampling were as follows:The sample use first mode HIV RNA NAT result is 32.4,the second mode HIV RNA NAT result is 32.69,the third mode HIV RNA NAT result is 32.76.The HIV RNA viral load result is 3.28×103IU/m L.three month later,CDC resample to completion of HIV confirmation expermation is HIV positive.Conclusion Test tube type has little influence on NAT results of HIV window period sample,Sufficient and no hemolysis and fat free blood samples from all three types of test tubes can be used for nucleic acid detection.

关 键 词：核酸检测 HIV 窗口期 NAT 

分 类 号：R446.6[医药卫生—诊断学]