人参皂苷Rg1抗长波紫外线对人皮肤成纤维细胞损伤模型的作用研究  

An Experimental Study on the Effects of Ginsenoside Rg1 on the Injury Model of Human Dermal Fibroblasts Induced by UVA

作　　者：周桃花 周红艳 陈芳 尹颖 杨玉 ZHOU Tao-hua;ZHOU Hong-yan;CHEN Fang;YIN Ying;YANG Yu(Department of Pharmacy,Sichuan Provincial Hospital for Women and Children,Affiliated Women and Children's Hospital of Chengdu Medical College,Chengdu 610041,Sichuan,China)

机构地区：[1]四川省妇幼保健院四川省妇女儿童医院成都医学院附属妇女儿童医院药学部,四川成都610041

出　　处：《中国美容医学》2021年第3期90-93,共4页Chinese Journal of Aesthetic Medicine

摘　　要：目的:观察人参皂苷Rg1抗长波紫外线(Ultravio1et A,UVA)对人皮肤成纤维细胞(Human dermal fibroblasts,HDF)的损伤作用,为研究开发光老化防护剂提供依据。方法:分离HDF进行原代培养及冻存;复苏HDF传代培养至第6~8代用于样品活性检测;采用改良MTT法检测UVA照射前后给予人参皂苷Rg1 HDF存活率的变化情况,用维生素C作阳性对照。结果:在照射前24h预先给予人参皂苷单体Rg1,在2.5~40μg/ml浓度时细胞存活率较模型组明显提高,差异有统计学意义(P<0.05);且5、10μg/ml组的细胞存活率优于阳性对照组(P<0.05)。照射后给予人参皂苷单体Rg1,在2.5~40μg/ml浓度时细胞存活率均较模型组明显提高(P<0.05)。结论:人参皂苷Rg1在2.5~40μg/ml范围内对HDF的UVA损伤有较好的预防和治疗作用。Objective T0 observe the anti-damage effect of ginsenoside Rg1 against ultraviolet A(UVA)on human dermal fibroblasts(HDF),and provide a basis for the research and development of photo aging protection agents.Methods Isolated HDF for primary culture and cryopreservation.Resuscitated HDF subculture to the 6th to 8th generation for sample activity detection.Utilizing the modified MTT method to detect the survival rate of HDF before and after UVA irradiation when treatment with ginsenoside Rg1,and vitamin C was used as a positive control.Results The ginsenoside monomer Rg1 was pre-administered 24h before irradiation,the cell survival rate was significantly improved compared with the model group at a concentration of 2.5-40μg/ml,the differences were statistically significant(P<0.05).And the cell survival rate of 5,10μg/ml group was better than vitamin C group(P<0.05).The ginsenoside Rg1 was given after irradiation,the cell survival rate at the concentration of 2.5-40μg/ml was significantly higher than those of the model group(P<0.05).Conclusion Ginsenoside Rg1 has a good preventive and therapeutic effect on the UVA damage ofHDF in the range of 2.5-40μg/ml.

关 键 词：人参皂苷RG1 人皮肤成纤维细胞 长波紫外线 光老化 预防 治疗 

分 类 号：R622[医药卫生—整形外科]