Successive digestion of tilapia collagen by serine proteinase and proline specific endopeptidase to produce novel angiotensin l-converting enzyme inhibitory peptides  

作　　者：Xin Hua Lechang Sun Chan Zhong Qiang Wu Panpan Xiao Asami Yoshida Guangming Liu Minjie Cao 

机构地区：[1]College of Food and Biological Engineering,Jimei University,Xiamen 361021,China [2]Collaborative Innovation Center of Marine Food Deep Processing,Dalian Polytechnic University,Dalian 116034,China [3]Department of Biological and Chemical Engineering,Shaoyang University,Shaoyang 422000,China [4]Faculty of Fisheries,Nagasaki University,Nagasaki 852-8521,Japan

出　　处：《Marine Life Science & Technology》2020年第3期268-278,共11页海洋生命科学与技术（英文）

基　　金：This work was sponsored by the National Key R&D Program of China(2018YFD0901004);the National Natural Scientific Foundations of China(31471640,31702372).

摘　　要：Serine proteinase,purified from the hepatopancreas of Pacific white shrimp(Litopenaeus vannamei), was used to hydrolyze acid solubilized collagen(ASC)isolated from Nile tilapia(Oreochromis sp.)skin to produce angiotensin I-converting enzyme(ACE)inhibitory peptides(ACEIPs).A series of column chromatography assays were used to separate the ACEIPs.A peptide,NPARTCR,was isolated as it exhibited high ACE inhibition potential.Further digestion of this peptide by a proline specific endopeptidase(PSEP),produced a pentapeptide ARTCR with ACE inhibitory activity(IC_(50))of 77.0 pmol/L.Both NPARTCR and ARTCR inhibited ACE in a non-competitive manner.An in vivo study in rats demonstrated that ARTCR has ACE inhibitory activity via lowering systolic blood pressure in spontaneously hypertensive rats(SHRs).These results suggest that processing by-products from shrimp and tilapia are ideal raw materials for the production of serine proteinase and collagen,respectively.Serine proteinase and collagen are both ideal raw materials that can be used to derive ACE inhibitory active peptides against hypertension.

关 键 词：Serine proteinase Purification COLLAGEN ACE inhibitory peptides Hypertension Lowering blood pressure 

分 类 号：S965.125[农业科学—水产养殖]