变应性鼻炎患儿中白介素-33高表达对Th2和Th17炎症反应的正向调控作用  

Positive Regulation of Interleukin-33 High Expression on Th2 and Th17 Inflammatory Response in Children with Allergic Rhinitis

作　　者：王远明[1] 李楚凌[1] 罗香林[1] 许德瑜[1] 徐亚雄[1] WANG Yuanming;LI Chuling;LUO Xianglin;XU Deyu;XU Yaxiong(Dongguan Women and Children’s Hospital,Dongguan 523000,China;不详)

机构地区：[1]广东省东莞市妇幼保健院,广东东莞523000

出　　处：《中国医学创新》2021年第6期10-14,共5页Medical Innovation of China

基　　金：2016年东莞市市属公立医院院长专项资金重点专科建设补助项目(23)。

摘　　要：目的:探讨白介素-33(IL-33)在变应性鼻炎患儿中的表达和对Th2和Th17炎症反应的调控作用。方法:选取2017年1-6月就诊于本院的50例变应性鼻炎患儿,根据是否合并哮喘分为变应性鼻炎组(n=40)与变应性鼻炎伴哮喘组(n=10),另选取同期于本院体检的20例正常儿童作为对照组。比较三组血清和鼻腔灌洗液中IL-33和Th2、Th17细胞因子的表达情况,分析变应性鼻炎患儿IL-33和Th相关细胞因子的相关性。纯化外周血单个核细胞进行IL-33的体外调节实验,分析重组IL-33和相关细胞因子对外周血单个核细胞Th2和Th17炎症反应的调控作用。结果:变应性鼻炎组与变应性鼻炎伴哮喘组中血清和鼻腔灌洗液IL-33、IL-4、IL-5、IL-17均高于对照组,且变应性鼻炎伴哮喘组均高于变应性鼻炎组(P<0.05)。变应性鼻炎患儿血清和鼻腔灌洗液中IL-4、IL-5、IL-17均与IL-33表达呈正相关(P<0.05)。血清ECP、粉尘螨特异性IgE水平及嗜酸性粒细胞计数均与血清IL-33表达呈正相关(P<0.05)。HDM和1、10、100 ng/mL rhIL-33的IL-4、IL-5、IL-17水平均高于PBS,且10、100 ng/mL rhIL-33均高于1 ng/mL rhIL-33(P<0.05)。100 ng/mL rhIL-33的IL-4、IL-5及IL-17水平均高于HDM(P<0.05)。HDM+anti-IL-33的IL-4、IL-5及IL-17水平均高于PBS,而低于HDM(P<0.05)。结论:变应性鼻炎中IL-33的表达上调促进了Th2和Th17炎症反应,并为IL-33在变应性鼻炎发病机制中的调节作用提供了可能的解释。Objective:To investigate the expression of interleukin-33(IL-33)in children with allergic rhinitis and its regulation of Th2 and Th17 inflammatory responses.Method:A total of 50 children with allergic rhinitis admitted to our hospital from January to June 2017 were selected,they were divided into allergic rhinitis group(n=40)and allergic rhinitis with asthma(n=10)according to whether they had asthma or not.Another 20 normal children underwent physical examination in our hospital during the same period were selected as the control group.The expression of IL-33 and Th2 and Th17 cytokines in serum and nasal lavage fluid of three groups were compared.The correlation between IL-33 and Th related cytokines in children with allergic rhinitis were analyzed.Purified peripheral blood mononuclear cells were used to conduct in vitro IL-33 regulation experiments,and the regulatory effects of recombinant IL-33 and related cytokines on Th2 and Th17 inflammatory response of peripheral blood mononuclear cells were analyzed.Result:The serum and nasal lavage fluid IL-33,IL-4,IL-5 and IL-17 in allergic rhinitis group and allergic rhinitis with asthma group were higher than those in the control group,and those in allergic rhinitis with asthma group were higher than those in allergic rhinitis group(P<0.05).The expression of IL-4,IL-5 and IL-17 in serum and nasal lavage fluid of children with allergic rhinitis were positively correlated with IL-33(P<0.05).Serum ECP,dust mite specific IgE levels and eosinophil count were positively correlated with serum IL-33 expression(P<0.05).The levels of IL-4,IL-5 and IL-17 in HDM and 1,10,100 ng/mL rhIL-33 were higher than those in PBS,and those in 10 and 100 ng/mL rhIL-33 were higher than those in 1 ng/mL rhIL-33(P<0.05).IL-4,IL-5 and IL-17 levels in 100 ng/mL rhIL-33 were higher than those in HDM(P<0.05).The levels of IL-4,IL-5 and IL-17 in HDM+anti-IL-33 were higher than those in PBS,but were lower than those in HDM(P<0.05).Conclusion:Up-regulated IL-33 protein in allergic rhinitis promote Th2 a

关 键 词：白介素-33 变应性鼻炎 儿童 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]