一株产(-)γ-内酰胺酶的Pantoea Ananatis Y22筛选及全细胞转化(+/-)γ-内酰胺  

Isolation of Pantoea Ananatis Y22 with (-) γ-Lactamase and WholeCell Resolution of Racemic γ-Lactam

作　　者：王金昌[1] 王建军[2] 靳亮[1] 占智高[1] 况文东 陈俊晖 关丽梅[1] WANG Jinchang;WANG Jianjun;JIN Liang;ZHAN Zhigao;KUANG Wendong;CHEN Junhui;GUAN Limei(Institute of Microbiology,Jiangxi Academy of Sciences,330096,Nanchang,PRC;Institute of Microbiology Chinese Academy of Sciences,100101,Beijing,PRC)

机构地区：[1]江西省科学院微生物研究所,南昌330096 [2]中国科学院微生物研究所,北京100101

出　　处：《江西科学》2021年第2期220-223,239,共5页Jiangxi Science

基　　金：江西省重点研发项目一般项目(20192BBF60056,20202BBFL63050);江西省科学院产学研项目(2020-YZD-13)。

摘　　要：利用(+/-)γ-内酰胺做为唯一碳源从土壤中分离出一株能产生(+)γ-内酰胺酶水解拆分(+/-)γ-内酰胺的菌株。鉴定菌株为Pantoea ananatis Y22。进一步优化了P.ananatis Y22发酵产γ-内酰胺酶的发酵条件和生物转化条件。发现棉子糖为最佳碳源,蛋白胨为最佳氮源;Y22在p H值4~10范围内生长良好,最佳发酵p H值为7,最佳生物转化p H值为8。确定最佳发酵温度为30℃,最佳生物转化温度为30℃。We isolated a strain that can produce(-)γ-lactamase to hydrolyze racemic(+/-)γ-lactam from several soil samples,using(+/-)γ-lactam as the only source of carbon.We identified this strain to be Pantoea ananatis Y22.We further optimized the enzyme fermentation and biotransformation conditions for theγ-lactamase from P.ananatis Y22.We found that glucose was the optimum carbon source and ammonium oxalate the optimal nitrogen source;P.ananatis Y22 showed good growth at pH values raning from 4 to 10,with an the optimum fermentation pH was 7,the optimum biotransformation pH was 8.The optimum fermentation temperature was determined to be 30℃and the optimal biotransformation temperature was 30℃.

关 键 词：(-)γ-内酰胺酶 (+)γ-内酰胺 生物转化 生物拆分 

分 类 号：Q936[生物学—微生物学]