红色毛癣菌、石膏样小孢子菌对人角质形成细胞TLR2/c-Jun、NF-κB信号通路的差异性诱导  

Differential induction of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by Trichophyton rubrum and Microsporum gypseum

作　　者：苏真 邓威威 湛舒婷 梁盼盼[1] 李美容[1] 尹颂超[1] 陈剑[1] SU Zhen;DENG Weiwei;ZHAN Shuting;LIANG Panpan;LI Meirong;YIN Songchao;CHEN Jian(The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China)

机构地区：[1]中山大学附属第三医院皮肤科,广东广州510630

出　　处：《皮肤性病诊疗学杂志》2021年第1期1-6,共6页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：国家自然科学基金(编号:81573058)。

摘　　要：目的:探讨不同生态学分类皮肤癣菌对人角质形成细胞TLR2/c-Jun、NF-κB信号通路的诱导。方法:将红色毛癣菌、石膏样小孢子菌分别与HaCaT细胞共培养24 h后,蛋白免疫印迹检测p65、relb、IκB、c-Jun的总蛋白及其磷酸化水平。然后将红色毛癣菌、石膏样小孢子菌分别与HaCaT细胞TLR2敲除细胞株(HaCaT TLR2-/-细胞)共培养24 h后,采用蛋白免疫印迹检测TLR2、c-Jun蛋白及其磷酸化水平。结果:HaCaT细胞感染红色毛癣菌后c-Jun蛋白磷酸化水平无明显变化,而感染石膏样小孢子菌后c-Jun蛋白磷酸化水平升高。p65、relb、IκB的总蛋白及其磷酸化水平在两种菌的感染下均无明显变化。HaCaT TLR2^(-/-)细胞感染红色毛癣菌后c-Jun蛋白磷酸化水平明显降低,而感染石膏样小孢子菌后c-Jun蛋白磷酸化水平无明显变化。结论:红色毛癣菌与石膏样小孢子菌都可以通过TLR2激活人角质形成细胞c-Jun信号通路,但并未激活NF-κB信号通路。此外,TLR2可能和其他受体共同介导了红色毛癣菌、石膏样小孢子菌对c-Jun信号通路的差异性诱导。Objective:To explore the activation of TLR2/c-Jun and NF-κB signaling pathway in human keratinocytes infected by different ecological classifications of dermatophytes.Methods:Total and phosphorylated p65,relb,IκB,and c-Jun in HaCaT cells were detected by immunoblotting following co-culture with either Trichophyton rubrum(T.rubrum)or Microsporum gypseum(M.gypseum)for 24 h.Immunoblotting was used to assess expression levels of TLR2,c-Jun,and phosphorylated c-Jun after co-culture of TLR2 deficient HaCaT cells(HaCaT TLR2^(-/-)cells)with T.rubrum and M.gypseum for 24 h.Results:Co-culture of HaCaT cells with M.gypseum,but not T.rubrum,significantly increased the levels of phosphorylated c-Jun.However,neither T.rubrum nor M.gypseum induced significant changes in the total and phosphorylated p56,relb,and IκB in HaCaT cells.Following TLR2 knockout in HaCaT cells,the level of phosphorylated c-Jun decreased significantly in HaCaT TLR2-/-cells co-cultured with T.rubrum,but not M.gypseum.Conclusions:T.rubrum and M.gypseum can activate c-Jun signaling pathway through TLR2 in keratinocytes,without activation of NF-κB signaling pathway.TLR2,together with other receptors,may dominate the differential induction of c-Jun pathway in keratinocytes infected by T.rubrum and M.gypseum.

关 键 词：角质形成细胞 红色毛癣菌 石膏样小孢子菌 TLR2 c-Jun信号通路 

分 类 号：R756[医药卫生—皮肤病学与性病学]