梅毒血清学试验阳性患者血清IL-17、IL-21水平与TRUST滴度相关性分析  

The relationship of serum IL-17 and IL-21 levels and TRUST titer in syphilis patients with positive serological test

作　　者：张荣 鲁东平[1,2] 王芬 贾婕[1,2] 张伟莲[1,2] 杨文志 李滨燕[1] 刘婷 ZHANG Rong;LU Dongping;WANG Fen;JIA Jie;ZHANG Weilian;YANG Wenzhi;LI Bingyan;LIU Ting(Department of Dermatology,Shenzhen Bao'an Traditional Chinese Medicine Hospital Group,Shenzhen 518133,China;Shenzhen Bao'an District Chronic Disease Hospital,Shenzhen 518133,China;Department of Obstetrics,Huangshi Maternity&Children's Health Hospital Edong Healthcare Group,Huangshi 435000,China)

机构地区：[1]深圳市宝安中医院(集团)皮肤科,广东深圳518133 [2]深圳市宝安区慢性病防治院皮肤科,广东深圳518133 [3]鄂东医疗集团黄石市妇幼保健院产科,湖北黄石435000

出　　处：《皮肤性病诊疗学杂志》2021年第1期7-11,共5页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：深圳市科技计划项目(编号:JCYJ20180302173806737);湖北省卫生健康委员会2019-2020年度面上项目(编号:WJ2019M045)。

摘　　要：目的:通过检测梅毒血清学试验阳性患者血清中IL-17、IL-21的表达水平,探讨梅毒血清固定形成的相关免疫机制。方法:随机选取30例血清梅毒固定患者作为梅毒血清固定组,以早期梅毒患者、驱梅治疗后TRUST阴转者和健康体检者各30例作为对照组,ELISA法检测血清IL-17、IL-21水平,对比组间白细胞介素水平差异;对早期梅毒患者、梅毒血清固定患者IL-17、IL-21水平与TRUST滴度关系行Pearson相关性分析。结果:早期梅毒组血清IL-17[(25.43±4.58)pg/mL]、IL-21[(36.14±6.54)pg/mL]水平最高,与其它三组比较差异均有统计学意义(P值均<0.05);梅毒血清固定组血清IL-17[(8.84±1.46)pg/mL]、IL-21[(16.52±5.84)pg/mL]水平低于健康对照组和治疗后TRUST转阴组,差异均有统计学意义(P值均<0.05);健康对照组[IL-17:(11.52±2.51)pg/mL;IL-21:(21.56±5.38)pg/mL]和治疗后TRUST转阴组[IL-17:(11.64±2.49)pg/mL;IL-21:(21.05±5.16)pg/mL]组间比较,差异无统计学意义(P值均>0.05)。早期梅毒组IL-17、IL-21水平与TRUST滴度正相关(r值分别为0.75、0.62,P值均<0.05);梅毒血清固定组的IL-17、IL-21水平与TRUST滴度无相关性(r值分别为0.22、0.15,P值均>0.05)。结论:早期梅毒患者体内的IL-17、IL-21参与了梅毒免疫过程;梅毒血清固定患者血清中IL-17、IL-21水平异常可能与血清固定的形成相关,但与TRUST滴度不相关。Objective:To explore the immune mechanism of syphilitic sero-fixation by measuring the expression levels of serum IL-17 and IL-21 in syphilis patients with positive serological test.Methods:Subjects included syphilitic sero-fixation,syphilis with Toluidine red unheated serum test(TRUST)negative,early syphilis,and normal healthy controls,30 subjects per groups.Serum concentrations of IL-17 and IL-21 were measured by ELISA,while TRUST titers were measured with TRUST kit.The differences in serum IL-17 and IL-21 levels were compared among four groups.Pearson correlation analysis was used to analyze the correlation between TRUST titers and IL-17 and IL-21 levels.Results:The serum levels of IL-17[(25.43±4.58)pg/mL]and IL-21[(36.14±6.54)pg/mL]in early syphilis group were significantly higher than that in the other three groups(P<0.05).The concentrations of serum IL-17[(8.84±1.46)pg/mL]and IL-21[(16.52±5.84)pg/mL]in syphilitic sero-fixation group were lower than those in both healthy controls[IL-17:(11.52±2.51)pg/mL;IL-21:(21.56±5.38)pg/mL]and the TRUST negative subjects[IL-17:(11.64±2.49)pg/mL;IL-21:(21.05±5.16)pg/mL](P<0.05),but were not comparable between the healthy controls and TRUST negative subjects(P>0.05).The serum levels of IL-17 and IL-21 were correlated positively with TRUST titers in the early syphilis patients(r=0.75,0.62,respectively,both P<0.05),but not in syphilitic sero-fixation patients(r=0.22,0.15,respectively,both P>0.05).Conclusions:Both IL-17 and IL-21 are involved in the syphilis immune process in early syphilis.The formation of syphilitic sero-fixation could be associated with decreased serum levels of IL-17 and IL-21,but not TRUST titers.

关 键 词：梅毒血清固定 白细胞介素-17 白细胞介素-21 甲苯胺红不加热血清实验 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]