丝棉木组培脱菌技术的研究  

Technology for Bacteria-free Euonymus maackii Seedling by Tissue Culture

作　　者：许丁帆 刘艳军 焦思鹏 黄俊轩 武春霞 李建科 XU Ding-fan;LIU Yan-jun;JIAO Si-peng;HUANG Jun-xuan;WU Chun-xia;LI Jian-ke(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300384,P.R.China)

机构地区：[1]天津农学院园艺园林学院,天津300384

出　　处：《西部林业科学》2021年第2期140-144,162,共6页Journal of West China Forestry Science

基　　金：天津市科技计划项目(18ZXBFNC00370);河北省产业创新创业团队项目(199A2905H);河北省创新能力提升计划项目(20566301D);河北省中央引导地方科技发展资金项目(206Z6303G)。

摘　　要：为研究增强丝棉木组培苗的抗逆性方法,以丝棉木1 a生休眠枝条带1~2个腋芽的茎段为外植体,经外植体表面消毒、腋芽萌发诱导并利用抗生素进行组培苗脱菌处理,研究不同抗生素种类、浓度及不同继代培养次数对丝棉木组培苗抑菌效果及其生长的影响,探索适合丝棉木组培脱菌的最佳方法。结果表明:1/2 MS+50mg/L制霉菌素+50mg/L硫酸链霉素+250mg/L羧苄青霉素钠能够有效抑制丝棉木外植体真菌与细菌污染,丝棉木外植体在该培养基上每隔20 d继代培养1次,3~4次继代培养后,并经生根培养后可获得生长势强的脱菌苗。In order to study the methods of enhancing the stress resistance of tissue culture seedlings of Euonymus maackii,the stem segments with 1-2 axillary buds of a-year-old dormant branches were used as explants.After surface disinfection of explants,axillary bud germination induction and antibiotic treatment,the effects of different antibiotic types,concentrations and subculture times on the antibacterial effect and growth of tissue culture seedlings of E.maackii were studied,and the best method suitable for tissue culture of E.maackii was explored.The results showed that the 1/2MS medium containing 50 mg/L nystatin,50 mg/L streptomycin sulfate and 250 mg/L carbenicillin disodium could effectively inhibit the explants of E.maackii in vivo fungus and bacterial contamination,and the explants of E.maackii were subcultured once every 20 days,and after 3 to 4 times of subculture and rooting culture,the strong growth potential of the bacteria-free seedlings could be obtained.

关 键 词：丝棉木 外植体 脱菌 组培苗 抗生素 

分 类 号：S723[农业科学—林木遗传育种]