Par3、p-JAK、p-STAT3蛋白在宫颈鳞癌组织中的表达及病理特点分析  

The expression and pathological features of par3, p-jak and p-stat3 proteins in cervical squamous cell carcinoma

作　　者：郭春凤[1] 黄修菊[1] 马俊旗[1] GUO Chunfeng;HUANG Xiuju;MA Junqi(Department of Gynecology,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院妇科,乌鲁木齐830054

出　　处：《新疆医科大学学报》2021年第4期448-452,共5页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区自然科学基金面上项目(2017D01C304)。

摘　　要：目的探讨极性蛋白Par3(partioning defective protein 3, Par3)、磷酸化的酪氨酸激酶(pJAK)、磷酸化的信号传导子和转录激活子3(p-STAT3)蛋白在慢性宫颈炎(chronic cervicitis,CC)、宫颈上皮内瘤变(cervical icalintfaepithelial neoplasia Ⅱ-Ⅲ,CIN Ⅱ-Ⅲ)和宫颈鳞状细胞癌(cervical squamous carcinoma,CSCC)、转移淋巴结组织中的表达特征及其与宫颈鳞癌的关系。方法选择新疆医科大学第一附属医院妇科2016年1月-2017年12月收治的因CINⅡ-Ⅲ、CSCC以及良性疾病行子宫切除术患者,调取存档石蜡包埋组织共93例,其中CC 14例,CINⅡ-Ⅲ36例,CSCC 43例。另选取CSCC组中7例阳性淋巴结组织标本。应用免疫组织化学染色法测定Par3、p-JAK和p-STAT3蛋白在CC、CIN Ⅱ-Ⅲ、CSCC及阳性淋巴结组织中的表达;分析三者与宫颈鳞癌临床病理参数关系;探讨三者在上述组织表达的相关性。结果 Par3在CSCC中的阳性表达率低于CC和CIN(P<0.05),p-JAK在CSCC中的阳性表达率高于CC,低于CIN(P<0.05),p-STAT3在CSCC中的阳性表达率高于CC(P<0.05)。Par3低表达与肿瘤组织学分化、淋巴结转移有关;p-JAK、p-STAT3表达上调与肿瘤临床分期、组织学分化有关(P<0.05)。CINⅡ-Ⅲ组织中Par3与p-JAK、Par3与p-STAT3蛋白表达关系均呈负相关(r=-5.553、-6.092、, P均<0.05);(r=-6.092, P<0.05)。结论宫颈鳞癌中Par3蛋白低表达,p-JAK、p-STAT3蛋白高表达上调,与宫颈鳞癌发病机制有关。Objective The expressions of par3,phosphorylated tyrosine kinase(p-jak)and phosphorylated signal transduction and transcriptional activator protein 3(p-stat3)were determined to analyze their relationship with chronic cervicitis(CC),cervical intraepithelial neoplasm(CIN),and cervical squamous cell carcinoma(CSCC).Methods From January 2016 to December 2017,paraffin-embedded tissue of 93 cases were collected,including 14 cases of CC,36 cases of CINⅡ-Ⅲ,43 cases of CSCC.In addition,7 positive lymph node tissue specimens from the CSCC group wereselectedand.The expression of PAR3,p-JAK and p-STAT3 proteins in CC,CINⅡ-Ⅲ,CSCC and positive lymphnodes were determined by immunohistochemical staining.The relationship between the three factors and clinicopatho-logical parameters of cervical squamous cell carcinoma was analyzed Results The positive expression of par3 inCSCC was lower than CC and CIN(p<0.05);the positive expression rate of p-JAK in CSCC was higher than that in CC,but lower than that in CIN(p<0.05);the positive expression rate of p-STAT3 in CSCC was higher than that in CC(p<0.05).Low expression of par3 in cervical cancer was associated with histological differentiation and lymph node metas-tasis(p<0.05).The high expression of p-jak and p-stat3 was related to the clinical stage and histological differentia-tion of the tumor(p<0.05).In CINⅡ-Ⅲ group,par3 was negatively correlated with the expression levels of p-jak(r=-5.553,P<0.05)or p-stat3(r=-6.092,P<0.05),respectively.Conclusion The down-regulation of par3 and the highexpression of p-jak and p-stat3 protein are related to the pathogenesis of cervical squamous cell carcinoma.

关 键 词：宫颈鳞癌 Par3蛋白 磷酸化的酪氨酸激酶 磷酸化的信号传导子和转录激活子3 

分 类 号：R714.21[医药卫生—妇产科学]