感染白粉病烟株典型病级叶际真菌群落结构与多样性分析  

Community structure and diversity of phyllosphere fungi in tobacco plants with typically infected tobacco powdery mildew

作　　者：黄宇 汪汉成 陈乾丽 陈兴江 向立刚 李忠[1] 谢红炼 HUANG Yu;WANG Hancheng;CHEN Qianli;CHEN Xingjiang;XIANG Ligang;LI Zhong;XIE Honglian(College of Agriculture,Guizhou University,Guiyang 550025,China;Guizhou Academy of Tobacco Science,Guiyang 550081,China;College of Life Sciences,Yangtze University,Jingzhou 434025,Hubei,China;College of Agriculture,Yangtze University,Jingzhou 434025,Hubei,China)

机构地区：[1]贵州大学农学院,贵阳市550025 [2]贵州省烟草科学研究院,贵阳市550081 [3]长江大学生命科学学院,湖北省434025 [4]长江大学农学院,湖北省荆州市434025

出　　处：《烟草科技》2021年第4期8-14,共7页Tobacco Science & Technology

基　　金：国家烟草专卖局科技项目“烟草主要叶斑类病害监测预警及绿色防控技术研究与应用”[110202001035(LS-04)];中国烟草总公司贵州省公司科技项目“烟草主要叶斑类病害监测预警及绿色防控技术研究与应用”(2020XM22)和“烟草青枯菌与烟草疫霉协同侵染烟草的微生态研究”(201914)。

摘　　要：为明确感染白粉病烟株的典型病级烟叶叶际真菌群落结构与多样性的变化,在同一烟株上采集3级病级烟叶(BFn)和9级病级烟叶(BFl)样品,采用Illumina MiSeq测序技术对叶际真菌群落进行高通量测序,并分析了各样品的真菌群落结构与多样性。结果表明:(1)感染白粉病3级病叶真菌群落丰富度高于9级病叶;(2)在门水平上,所有样品的优势菌门为子囊菌门(Ascomycota)和担子菌门(Basidiomycota);(3)在属水平上,3级病叶真菌种类远多于9级病叶,3级病叶主要菌属为高氏白粉菌属(Golovinomyces,相对丰度99.47%)、曲霉属(Aspergillus,0.16%)、红酵母属(Rhodotorula,0.13%)、链格孢属(Alternaria,0.08%);9级病叶主要菌属为高氏白粉菌属(99.43%)、曲霉属(0.54%)和链格孢属(0.01%)。因此,3级和9级病叶主要优势菌属均为高氏白粉菌属,其丰度受病情指数的影响较小。In order to clarify the variations in community structure and diversity of phyllosphere fungi in tobacco plants with typical infection of tobacco powdery mildew,the tobacco leaves with disease severityⅢ(BFn)and severity IX(BFl)were sampled from the same tobacco plant,and the fungal community structure and diversity of the samples were investigated by using Illumina high-throughput sequencing technique.The results showed that:(1)The fungal community abundance in the BFn sample were higher than that of the BFl sample.(2)At the phylum level,the dominant fungi of all the samples were Ascomycota and Basidiomycota.(3)At the genus level,BFn had more diversity than BFl.The dominant genuses of BFn were Golovinomyces(the relative abundance 99.47%),Aspergillus(0.16%),Rhodotorula(0.13%)and Alternaria(0.08%),while for BFl they were Golovinomyces(99.43%),Aspergillus(0.54%)and Alternaria(0.01%).Therefore,Golovinomyces were the dominant bacterial genus for both BFn and BFl,and its abundance was less affected by disease index.

关 键 词：烟草白粉病 Illumina高通量测序 真菌多样性 群落结构 高氏白粉菌 

分 类 号：S43[农业科学—农业昆虫与害虫防治]