UPLC法比较黄芩不同炮制品中5种黄酮类成分  

UPLC-based comparison of five flavonoids in different processed products of Scutellaria baicalensis

作　　者：罗兰 魏劭恒 张英[2,3] 吴孟华[2,3] 曹晖[2,3] 马志国[2,3] LUO Lan;WEI Shao-heng;ZHANG Ying;WU Meng-hua;CAO Hui;MA Zhi-guo(College of Pharmacy,Xinhua College of Sun Yat-sen University,Guangzhou 510520,China;College of Pharmacy,Jinan University,Guangzhou 510632,China;Research Center for TCM of Lingnan,Jinan University,Guangzhou 510632,China)

机构地区：[1]中山大学新华学院药学院,广东广州510520 [2]暨南大学药学院,广东广州510632 [3]暨南大学岭南传统中药研究中心,广东广州510632

出　　处：《中成药》2021年第4期966-970,共5页Chinese Traditional Patent Medicine

摘　　要：目的建立UPLC法比较生黄芩、酒黄芩、黄芩炭中5种黄酮类成分的含量。方法各样品溶液的分析采用Acquity UPLC HSS T3色谱柱(2.1 mm×100 mm,1.8μm);流动相乙腈-0.5%甲酸,梯度洗脱;体积流量0.3 mL/min;检测波长280 nm。结果5种黄酮类成分在各自范围内线性关系良好(r>0.9999),平均加样回收率100.17%~106.00%,RSD 2.00%~3.37%。黄芩酒炙后,野黄芩苷、黄芩苷和汉黄芩苷3种黄酮苷含量均有所下降,而黄芩素和汉黄芩素2种黄酮苷元含量有所增加;黄芩炒炭后,3种黄酮苷的含量下降更为明显,而2种黄酮苷元的含量明显增加。结论该方法准确稳定,重复性好,可用于黄芩的质量控制。AIM To establish a UPLC method for comparing content of five flavonoids in crude Scutellariae Radix,wine-processed Scutellariae Radix and charred Scutellariae Radix.METHODS The analysis of various samples was performed on a thermostatic Acquity UPLC HSS T3 column(2.1 mm×100 mm,1.8μm),with the mobile phase comprising of acetonitrile-0.5%formic acid flowing at 0.3 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.RESULTS Five flavonoids showed good linear relationships with-in their own ranges(r>0.9999),whose average recoveries were 100.17%-106.00%,with the RSDs of 2.00%-3.37%.Wine processing brought forth decreased contents of three flavonoid glycosides,scutellarin,baicalin and wogonoside;but increased contents of two flavonoid aglydons,baicalein and wogonin.Carbonizing process signifi-cantly decreased the contents of three flavonoid glycosides,and sharply increased the contents of two flavonoid agly-cones.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of Scutellaria baicalensis Georgi.

关 键 词：黄芩 生黄芩 酒黄芩 黄芩炭 黄酮 UPLC 

分 类 号：R284.1[医药卫生—中药学]