Genome-wide association study identifies novel loci and candidate genes for drought stress tolerance in rapeseed  

作　　者：Ali Shahzad Minchao Qian Bangyang Sun Umer Mahmood Shengting Li Yonghai Fan Wei Chang Lishi Dai Hong Zhu Jiana Li Cunmin Qu Kun Lu 

机构地区：[1]College of Agronomy and Biotechnology,Southwest University,Chongqing,400715,China [2]Academy of Agricultural Sciences,Southwest University,Chongqing,400715,China [3]Engineering Research Center of South Upland Agriculture,Ministry of Education,Chongqing,400715,China [4]College of Life Sciences,Yangtze University,Jingzhou,434025,Hubei,China

出　　处：《Oil Crop Science》2021年第1期12-22,共11页中国油料作物学报(英文版）

基　　金：supported by grants from the National Key Research and Development Plan(2018YFD0100500);the National Natural Science Foundation of China(31871653);the Fundamental Research Funds for the Central Universities(XDJK2020D023);the Chongqing Postgraduate Research and Innovation Projects(CYS20124);the Natural Science Foundation of Chongqing,China(cstc2018jcyjAX0347);the 111 project(B12006).

摘　　要：Rapeseed(Brassica napus)is one of the most important oil crops worldwide;however,drought seriously curtails its growth and productivity.Identifying drought-tolerant germplasm is an efficient strategy for addressing water shortages.Here,we phenotyped a panel of 264 B.napus accessions at full-bloom stage using water loss ratio(WLR)as drought-tolerant index.It identified 8 low-WLR and 6 high-WLR accessions,regarded as droughttolerant and drought-sensitive,respectively.Comparing with drought-sensitive accessions at the seedling stage,drought-tolerant accessions had shown better performance in maintaining fresh and dry weights,and performed the higher expression of drought-induced marker genes under drought stress.Subsequently,a total of 139 SNPs(single nucleotide polymorphisms)were identified associated with the WLR using a genome-wide association study(GWAS)among 264 B.napus accessions,with the largest number SNPs at chromosome A10,and 13 SNPs significantly were associated with the WLR(-log_(10)(p-value)>6).Furthermore,4 putative candidate genes(BnaC09.RPS6,BnaC09.MATE,BnaA10.PPD5 and BnaC09.Histone)were screened involving in drought tolerance in B.napus.Together,our results highlight the WLR's importance in drought tolerance and establish the foundation for improving WLR-associated drought tolerance in rapeseed.

关 键 词：GWAS Water loss ratio DROUGHT SNP RAPESEED 

分 类 号：S565.4[农业科学—作物学]