微波辅助柚皮苷非水相酶促反应及对HepG2细胞的抗增殖影响  

Microwave-Assisted Enzymatic Preparation of Acylated Naringin in Non-aqueous and Its Anti-Proliferation Effect on HepG2 Cells

作　　者：姜丽艳[1] 赵博[1] 王智[2] 陈启瑞 李玥莹 闫国栋[1] JIANG Liyan;ZHAO Bo;WANG Zhi;CHENG Qirui;LI Yueying;YAN Guodong(College of Life Science,Jilin University,Changchun 130023,China;Key laboratory for Molecular Enzymology&Engineering the Ministry of Education,Jilin University,Changchun 130023,China)

机构地区：[1]吉林大学生命科学学院,吉林长春130012 [2]吉林大学分子酶学工程教育部重点实验室,吉林长春130012

出　　处：《化学反应工程与工艺》2020年第1期90-96,共7页Chemical Reaction Engineering and Technology

基　　金：国家自然科学基金面上项目(21874054);吉林省科技发展计划项目(20200301029RQ)。

摘　　要：为了提高柚皮苷的稳定性及其生理活性,利用微波辅助非水相酶催化反应完成柚皮苷的乙酰化合成。考察并优化了影响柚皮苷微波酶促乙酰化反应的主要因素,以及其对HepG2细胞的抗增殖影响。结果表明,微波功率为400W、微波温度为70℃、水活度为0.80时柚皮苷酶促乙酰化反应的最高酶活为(612±10)μmol/(h·g),比传统方法提高4倍左右,且在2h内的转化率为90.42%±2.43%,经核磁波谱仪鉴定反应产物为6′-乙酰化柚皮苷。虽与母体柚皮苷相比,乙酰化柚皮苷的体外抗氧化活性略微降低,但对HepG2细胞具有较强的抗增殖能力。表明,柚皮苷非水相酶促乙酰化反应具有生物学意义。Acetylated naringin was synthesized by microwave-assisted non-aqueous enzymatic reaction to improve the stability and physiological bioactivity.The effects of various parameters on the enzyme activity and anti-proliferation effect on HepG2 were investigated and optimized.The results showed that the enzyme activity reached(612±10)μmol(/h·g)under the optimum conditions of microwave power 400 W,microwave temperature 70℃and water activity 0.80,which was about 4 times higher compared to traditional reaction.The maximum conversion was(90.42±2.43)%after reaction 2 h.The product was identified as 6’-acetylnaringin by nuclear magnetic resonance(NMR),the mass fraction was larger than 99%.Although the antioxidant activity of acetylated naringin in vitro was slightly lower than that of maternal naringin,it had a strong antiproliferative activity against HepG2 cells.That meant that the non-aqueous enzymatic acetylation of naringin has biological significance.

关 键 词：柚皮苷 非水相酶促反应 乙酰化 抗氧化 微波强化 

分 类 号：TQ460.1[化学工程—制药化工]