2011-2019年33株人源非O157产志贺毒素大肠埃希菌耐药表型及耐药相关基因分析  

Antimicrobial resistance in 33 non-O157 Shiga toxin-producing Escherichia coli isolated from human cases from 2011 to 2019

作　　者：潘衍宇 杨茜 曹丽娇 刘倩 熊衍文[1] Pan Yanyu;Yang Xi;Cao Lijiao;Liu Qian;Xiong Yanwen(State Key Laboratory of Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《中华预防医学杂志》2021年第3期365-370,共6页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金(81772152)。

摘　　要：目的了解人源非O157产志贺毒素大肠埃希菌(STEC)抗生素耐药表型及耐药基因携带状况。方法33株非O157 STEC菌株来自2011-2019年7个省份,包括青海、黑龙江(各1例),广西、山东(各2例),广东(4例),河南(11例),上海(12例),均分离自感染性腹泻患者粪便标本。采用改良微量肉汤法对非O157 STEC进行19种抗生素最小抑菌浓度(MIC)检测;通过全基因组测序进行O∶H血清分型、多位点序列分型(MLST)及耐药相关基因预测。结果33株非O157 STEC分为19种O∶H血清型、17种MLST序列型;10株菌对1种或以上抗生素耐药,5株为多重耐药菌株,对四环素耐药率最高(30.3%,10株),并检出阿奇霉素耐药菌株(12.1%,4株),但所有菌株均对碳青霉烯类药物敏感;共检出22种耐药基因,全部菌株均携带blaEC基因,并检出超广谱β-内酰胺酶(ESBL)基因型blaCTX-M-15(3.0%,1株),首次检出fosA7基因。结论中国7省份33株人源非O157 STEC中存在多重耐药和阿奇霉素耐药菌株,且携带多种耐药基因型,但均对碳青霉烯类药物敏感。Objective To evaluate the antimicrobial resistance of non-O157 Shiga toxin-producing Escherichia coli(STEC)isolated from human cases.Methods From 2011 to 2019,33 non-O157 STEC strains recovered from diarrheal patients from 7 provinces/cities were collected,including Qinghai(1 isolate),Heilongjiang(1 isolate),Guangxi(2 isolates),Shandong(2 isolates),Guangdong(4 isolates),Henan(11 isolates),and Shanghai(12 isolates).Minimum inhibitory concentration(MIC)of 19 antimicrobials were tested by broth microdilution method;O∶H serotypes,Multi-locus sequence typing(MLST)and antimicrobial resistance genes were determined by whole genome sequencing.Results A total of 33 non-O157 STEC strains were typed into 19 O∶H serotypes and 17 sequence types(STs),respectively.Ten strains were resistant to one or more antibiotics,of which five were multiple drug-resistant(MDR).The resistance rate of tetracycline was 30.3%(10 isolates),and azithromycin resistant strains were detected(12.12%,4 isolates),but all strains were susceptible to carbapenems.All strains carried the blaEC gene,and the Extended-Spectrumβ-Lactamase(ESBL)genotype blaCTX-M-15 were detected(3.0%,1 isolates).The fosA7 gene was firstly detected in non-O157 STEC strains.Conclusion MDR,azithromycin resistance,and multiple drug resistance genes were detected in human-derived non-O157 STECs in many regions in China,but they were all susceptible to carbapenems.Our results might guide the clinical treatment of STEC infections.

关 键 词：大肠埃希菌 志贺毒素 耐药表型 超广谱Β-内酰胺酶 

分 类 号：R446.5[医药卫生—诊断学]